J Cao1, L Teng, X Cai. 1. Cancer Institute, Zhejiang Medical University, Hangzhou.
Abstract
OBJECTIVE: Inhibition effect of p53 antisense RNA on malignant phenotype of colorectal cancer cells was studied. METHODS: For the purpose of inhibiting tumorigenecity of mutant p53 gene in colorectal cancer cells, a 2.1 kb human p53 full length cDNA was inserted into a mammalian expression vector pREP9 to make a p53 antisense RNA expression vector pREP9-p53 (AS). pREP9-p53 (AS) was then introduced into human colorectal cancer cell line SW1116 (with mutated endogenous p53). MTT method and FCM analysis were performed to measure the effect of p53 antisense RNA expressed by pREP9-p53 (AS) on SW1116 cell cycle progression. RESULTS AND CONCLUSION: It was shown that the growth rate of SW1116 cells with pREP9-p53 (AS) was significantly suppressed by the expression of p53 antisense RNA as compared to the control SW1116 cells and SW1116 cells with pREP9 vector alone. FCM analysis showed that SW1116 cells with pREP9-p53 (AS) were arrested at G0/1 phase, whereas no significant influence can be observed on control SW1116 cells with pREP9.
OBJECTIVE: Inhibition effect of p53 antisense RNA on malignant phenotype of colorectal cancer cells was studied. METHODS: For the purpose of inhibiting tumorigenecity of mutant p53 gene in colorectal cancer cells, a 2.1 kb humanp53 full length cDNA was inserted into a mammalian expression vector pREP9 to make a p53 antisense RNA expression vector pREP9-p53 (AS). pREP9-p53 (AS) was then introduced into humancolorectal cancer cell line SW1116 (with mutated endogenous p53). MTT method and FCM analysis were performed to measure the effect of p53 antisense RNA expressed by pREP9-p53 (AS) on SW1116 cell cycle progression. RESULTS AND CONCLUSION: It was shown that the growth rate of SW1116 cells with pREP9-p53 (AS) was significantly suppressed by the expression of p53 antisense RNA as compared to the control SW1116 cells and SW1116 cells with pREP9 vector alone. FCM analysis showed that SW1116 cells with pREP9-p53 (AS) were arrested at G0/1 phase, whereas no significant influence can be observed on control SW1116 cells with pREP9.