X Wang1, B Xu. 1. Shanghai Institute of Materia Medica, Chinese Academy of Sciences.
Abstract
OBJECTIVE: To study the antitumor and immunological activities of oxalysine (OXL). METHODS: Growth of mouse ascites hepatoma-22 (H-22) cells and splenocyte proliferation were investigated in vitro by MTT colorimetric assay. RESULTS: Suppression of OXL (6.3-200 micrograms/ml) on H-22 cell growth was weaker when used singly. But a marked synergetic inhibition was observed when OXL was used in combination with 5-fluorouracil (5-Fu), with suppression percentage on hepatoma growth increased to 43.1% from 13.8% (25 micrograms/ml of OXL alone) or 18.9% (3.1 micrograms/ml of 5-Fu alone). OXL (6.3-100 micrograms/ml) in vitro produced an inhibitory action on splenocyte proliferation induced by concanvilian A (Con A, 5 micrograms/ml) in the normal mice, but was able to enhance obviously the proliferative response in mice bearing H-22. CONCLUSION: OXL inhibits H-22 cell growth. The immunoregulatory action of OXL may contribute to its antihepatoma activity.
OBJECTIVE: To study the antitumor and immunological activities of oxalysine (OXL). METHODS: Growth of mouseascites hepatoma-22 (H-22) cells and splenocyte proliferation were investigated in vitro by MTT colorimetric assay. RESULTS: Suppression of OXL (6.3-200 micrograms/ml) on H-22 cell growth was weaker when used singly. But a marked synergetic inhibition was observed when OXL was used in combination with 5-fluorouracil (5-Fu), with suppression percentage on hepatoma growth increased to 43.1% from 13.8% (25 micrograms/ml of OXL alone) or 18.9% (3.1 micrograms/ml of 5-Fu alone). OXL (6.3-100 micrograms/ml) in vitro produced an inhibitory action on splenocyte proliferation induced by concanvilian A (Con A, 5 micrograms/ml) in the normal mice, but was able to enhance obviously the proliferative response in mice bearing H-22. CONCLUSION:OXL inhibits H-22 cell growth. The immunoregulatory action of OXL may contribute to its antihepatoma activity.