J Stap1, J Van Marle, H A Van Veen, J A Aten. 1. Department of Cell Biology and Histology, Center for Microscopical Research, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands. j.stap@amc.uva.nl
Abstract
BACKGROUND: For high-resolution microscopy, cells have to be analyzed through thin glass coverslips. Therefore, it is necessary to culture cells on coverslips for preservation of cell morphology. We found cell attachment and spreading to be relatively slow processes, even when cells were plated on coated coverslips. This slowness presents a problem, particularly when synchronized cell populations are used. METHODS: In this paper, we present a method that is based on glow-discharged carbon coating of coverslips which promotes rapid attachment and spreading of cells, enabling rapid analysis of cells after plating. Results obtained with carbon-coated coverslips were compared with those of other types of coating. Two fibroblast lines, an epithelial cell line, and a carcinoma cell line were tested. RESULTS AND CONCLUSIONS: All cell lines showed a rapid adhesion on carbon-coated coverslips. With fibroblasts we found the carbon coating to be superior to other coatings tested, mainly because the carbon did not influence cell morphology. Using synchronized or irradiated cells produced similar results. The superior performance of carbon coating is probably due to carboxylic groups on the glow-discharged carbon layer. The carbon layer does not interfere with microscopy or immunocytochemical staining procedures. Copyright 2000 Wiley-Liss, Inc.
BACKGROUND: For high-resolution microscopy, cells have to be analyzed through thin glass coverslips. Therefore, it is necessary to culture cells on coverslips for preservation of cell morphology. We found cell attachment and spreading to be relatively slow processes, even when cells were plated on coated coverslips. This slowness presents a problem, particularly when synchronized cell populations are used. METHODS: In this paper, we present a method that is based on glow-discharged carbon coating of coverslips which promotes rapid attachment and spreading of cells, enabling rapid analysis of cells after plating. Results obtained with carbon-coated coverslips were compared with those of other types of coating. Two fibroblast lines, an epithelial cell line, and a carcinoma cell line were tested. RESULTS AND CONCLUSIONS: All cell lines showed a rapid adhesion on carbon-coated coverslips. With fibroblasts we found the carbon coating to be superior to other coatings tested, mainly because the carbon did not influence cell morphology. Using synchronized or irradiated cells produced similar results. The superior performance of carbon coating is probably due to carboxylic groups on the glow-discharged carbon layer. The carbon layer does not interfere with microscopy or immunocytochemical staining procedures. Copyright 2000 Wiley-Liss, Inc.
Authors: Ineke D C Jansen; Jenny A F Vermeer; Veerle Bloemen; Jan Stap; Vincent Everts Journal: Calcif Tissue Int Date: 2012-04-25 Impact factor: 4.333
Authors: Jakub A Kochan; Matthias van den Belt; Julia von der Lippe; Emilie C B Desclos; Barbara Steurer; Ron A Hoebe; Enzo M Scutigliani; Jan Verhoeven; Jan Stap; Ruben Bosch; Meindert Rijpkema; Carel van Oven; Henk A van Veen; Irene Stellingwerf; Lianne E M Vriend; Jurgen A Marteijn; Jacob A Aten; Przemek M Krawczyk Journal: Nucleic Acids Res Date: 2019-09-26 Impact factor: 16.971
Authors: Michaela Wenzel; Marien P Dekker; Biwen Wang; Maroeska J Burggraaf; Wilbert Bitter; Jan R T van Weering; Leendert W Hamoen Journal: Commun Biol Date: 2021-03-08