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Literature DB >> 10737323

A new technique distinguishing alpha2-3 sialyl linkage from alpha2-6 linkage in sialyllactoses and sialyl-N-acetyllactosamines by post-source decay fragmentation method of MALDI-TOF mass spectrometry.

Abstract

Alpha2-3 and alpha2-6 sialyl linkage types of sialyllactoses and sialyl-N-acetyllactosamines were analyzed by post-source decay (PSD) fragmentation method using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. A new matrix of norharmane was suited for the MALDI-TOF measurements of sialyl oligosaccharides. The fragment ions B1 produced by the cleavage of alpha2-3 sialyl linkages indicate much higher intensity than those produced by the cleavage of alpha2-6 sialyl linkages in sialyllactoses and sialyl-N-acetyllactosamines. Thus, alpha2-3 sialyl linkages cleave much easier than alpha2-6 sialyl linkages in MALDI-PSD fragmentation method. These results suggest that the new techniques using PSD fragmentation of MALDI-TOF mass spectrometry enables us to distinguish alpha2-3 sialyl linkage from alpha2-6 linkage in sialyl oligosaccharides.

Entities: Chemical Gene

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Year: 1999 PMID： 10737323 DOI： 10.1023/a:1007050909293

Source DB: PubMed Journal: Glycoconj J ISSN： 0282-0080 Impact factor: 2.916

10 in total

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Journal: Carbohydr Res Date: 1991-12-16 Impact factor: 2.104

2. Analysis of glycosidic linkages in saccharide compounds by post-source decay fragment methods in matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy.

Authors: T Yamagaki; Y Ishizuka; S Kawabata; H Nakanishi
Journal: Rapid Commun Mass Spectrom Date: 1997 Impact factor: 2.419

3. Sialidase of swine influenza A viruses: variation of the recognition specificities for sialyl linkages and for the molecular species of sialic acid with the year of isolation.

Authors: G Xu; T Suzuki; Y Maejima; T Mizoguchi; M Tsuchiya; M Kiso; A Hasegawa; Y Suzuki
Journal: Glycoconj J Date: 1995-04 Impact factor: 2.916

4. A curved field reflectron time-of-flight mass spectrometer for the simultaneous focusing of metastable product ions.

Authors: T J Cornish; R J Cotter
Journal: Rapid Commun Mass Spectrom Date: 1994-09 Impact factor: 2.419

5. Post-source decay fragment spectra of cyclomalto-octaose and branched cyclomalto-hexaose by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

Authors: T Yamagaki; Y Ishizuka; S Kawabata; H Nakanishi
Journal: Rapid Commun Mass Spectrom Date: 1996 Impact factor: 2.419

6. Influence of different glycosidic linkages on relative ion intensities in post-source decay fragmentation of a xyloglucan heptaoligosaccharide using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

Authors: T Yamagaki; Y Mitsuishi; H Nakanishi
Journal: Rapid Commun Mass Spectrom Date: 1998 Impact factor: 2.419

7. beta-Carboline alkaloids as matrices for UV-matrix-assisted laser desorption/ionization time-of-flight mass spectrometry in positive and negative ion modes. Analysis of proteins of high molecular mass, and of cyclic and acyclic oligosaccharides.

Authors: H Nonami; K Tanaka; Y Fukuyama; R Erra-Balsells
Journal: Rapid Commun Mass Spectrom Date: 1998 Impact factor: 2.419

8. Determination of the sialylation level and of the ratio alpha-(2-->3)/alpha-(2-->6) sialyl linkages of N-glycans by methylation and GC/MS analysis.

Authors: D Cointe; Y Leroy; F Chirat
Journal: Carbohydr Res Date: 1998-09 Impact factor: 2.104

9. Structural analysis of xyloglucan oligosaccharides by the post-source decay fragmentation method of MALDI-TOF mass spectrometry: influence of the degree of substitution by branched galactose, xylose, and fucose on the fragment ion intensities.

Authors: T Yamagaki; Y Mitsuishi; H Nakanishi
Journal: Biosci Biotechnol Biochem Date: 1998-12 Impact factor: 2.043

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Authors: D I Papac; A Wong; A J Jones
Journal: Anal Chem Date: 1996-09-15 Impact factor: 6.986