Literature DB >> 10733881

Expression, purification, and characterization of histidine-tagged mouse monoglyceride lipase from baculovirus-infected insect cells.

M Karlsson1, H Tornqvist, C Holm.   

Abstract

Monoglyceride lipase (MGL) has been produced with the baculovirus-insect cell system. The mouse MGL cDNA was subcloned into a baculovirus transfer vector in frame with a sequence encoding an N-terminal stretch of six histidine residues. Purification to apparent homogeneity was obtained by nickel-chelating chromatography. The final yield was 3 mg of pure enzymatically active MGL per liter of Sf9 cell suspension culture. Analysis by SDS-PAGE and mass spectrometry showed that the recombinant histidine-tagged enzyme had the expected molecular mass. With monoolein as substrate, the specific activity and the apparent K(m) were close to those of rat MGL of adipose tissue. Copyright 2000 Academic Press.

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Year:  2000        PMID： 10733881     DOI： 10.1006/prep.1999.1194

Source DB:  PubMed          Journal:  Protein Expr Purif        ISSN： 1046-5928            Impact factor:   1.650


  12 in total

Review 1.  A review of the interactions between alcohol and the endocannabinoid system: implications for alcohol dependence and future directions for research.

Authors:  Matthew J Pava; John J Woodward
Journal:  Alcohol       Date:  2012-03-27       Impact factor: 2.405

2.  Inhibition of monoacylglycerol lipase and fatty acid amide hydrolase by analogues of 2-arachidonoylglycerol.

Authors:  Nazdar Ghafouri; Gunnar Tiger; Raj K Razdan; Anu Mahadevan; Roger G Pertwee; Billy R Martin; Christopher J Fowler
Journal:  Br J Pharmacol       Date:  2004-10-18       Impact factor: 8.739

3.  Crystal structure of a soluble form of human monoglyceride lipase in complex with an inhibitor at 1.35 Å resolution.

Authors:  Céline Schalk-Hihi; Carsten Schubert; Richard Alexander; Shariff Bayoumy; Jose C Clemente; Ingrid Deckman; Renee L DesJarlais; Keli C Dzordzorme; Christopher M Flores; Bruce Grasberger; James K Kranz; Frank Lewandowski; Li Liu; Hongchang Ma; Diane Maguire; Mark J Macielag; Mark E McDonnell; Tara Mezzasalma Haarlander; Robyn Miller; Cindy Milligan; Charles Reynolds; Lawrence C Kuo
Journal:  Protein Sci       Date:  2011-03-01       Impact factor: 6.725

4.  Full mass spectrometric characterization of human monoacylglycerol lipase generated by large-scale expression and single-step purification.

Authors:  Nikolai Zvonok; John Williams; Meghan Johnston; Lakshmipathi Pandarinathan; David R Janero; Jing Li; Srinivasan C Krishnan; Alexandros Makriyannis
Journal:  J Proteome Res       Date:  2008-05-02       Impact factor: 4.466

5.  Identification of Yju3p as functional orthologue of mammalian monoglyceride lipase in the yeast Saccharomycescerevisiae.

Authors:  Christoph Heier; Ulrike Taschler; Srinivasan Rengachari; Monika Oberer; Heimo Wolinski; Klaus Natter; Sepp D Kohlwein; Regina Leber; Robert Zimmermann
Journal:  Biochim Biophys Acta       Date:  2010-06-08

6.  Biochemical and pharmacological characterization of human α/β-hydrolase domain containing 6 (ABHD6) and 12 (ABHD12).

Authors:  Dina Navia-Paldanius; Juha R Savinainen; Jarmo T Laitinen
Journal:  J Lipid Res       Date:  2012-09-11       Impact factor: 5.922

7.  Characterization of an exported monoglyceride lipase from Mycobacterium tuberculosis possibly involved in the metabolism of host cell membrane lipids.

Authors:  Karen Côtes; Rabeb Dhouib; Isabelle Douchet; Henri Chahinian; Alain de Caro; Frédéric Carrière; Stéphane Canaan
Journal:  Biochem J       Date:  2007-12-15       Impact factor: 3.857

8.  Monoglyceride lipase deficiency in mice impairs lipolysis and attenuates diet-induced insulin resistance.

Authors:  Ulrike Taschler; Franz P W Radner; Christoph Heier; Renate Schreiber; Martina Schweiger; Gabriele Schoiswohl; Karina Preiss-Landl; Doris Jaeger; Birgit Reiter; Harald C Koefeler; Jacek Wojciechowski; Christian Theussl; Josef M Penninger; Achim Lass; Guenter Haemmerle; Rudolf Zechner; Robert Zimmermann
Journal:  J Biol Chem       Date:  2011-03-23       Impact factor: 5.157

9.  The structure of monoacylglycerol lipase from Bacillus sp. H257 reveals unexpected conservation of the cap architecture between bacterial and human enzymes.

Authors:  Srinivasan Rengachari; Gustavo A Bezerra; Lina Riegler-Berket; Christian C Gruber; Christian Sturm; Ulrike Taschler; Andras Boeszoermenyi; Ingrid Dreveny; Robert Zimmermann; Karl Gruber; Monika Oberer
Journal:  Biochim Biophys Acta       Date:  2012-04-27

10.  Conformational plasticity and ligand binding of bacterial monoacylglycerol lipase.

Authors:  Srinivasan Rengachari; Philipp Aschauer; Matthias Schittmayer; Nicole Mayer; Karl Gruber; Rolf Breinbauer; Ruth Birner-Gruenberger; Ingrid Dreveny; Monika Oberer
Journal:  J Biol Chem       Date:  2013-09-06       Impact factor: 5.157
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