Literature DB >> 10733742

A simple method for human peripheral blood monocyte isolation.

M C de Almeida1, A C Silva, A Barral, M Barral Netto.   

Abstract

We describe a simple method using percoll gradient for isolation of highly enriched human monocytes. High numbers of fully functional cells are obtained from whole blood or buffy coat cells. The use of simple laboratory equipment and a relatively cheap reagent makes the described method a convenient approach to obtaining human monocytes.

Entities:  

Mesh：

Year:  2000        PMID： 10733742     DOI： 10.1590/s0074-02762000000200014

Source DB:  PubMed          Journal:  Mem Inst Oswaldo Cruz        ISSN： 0074-0276            Impact factor:   2.743


  38 in total

1.  Functional activity of human monocytes exposed to lipopolysaccharides of different structure.

Authors:  M G Vinokurov; M M Yurinskaya; I R Prokhorenko; S V Prokhorenko; S V Grachev
Journal:  Dokl Biol Sci       Date:  2004 Mar-Apr

2.  Function of partially duplicated human α77 nicotinic receptor subunit CHRFAM7A gene: potential implications for the cholinergic anti-inflammatory response.

Authors:  Ana M de Lucas-Cerrillo; M Constanza Maldifassi; Francisco Arnalich; Jaime Renart; Gema Atienza; Rocío Serantes; Jesús Cruces; Aurora Sánchez-Pacheco; Eva Andrés-Mateos; Carmen Montiel
Journal:  J Biol Chem       Date:  2010-11-03       Impact factor: 5.157

3.  Exogenous heat shock protein HSP70 suppresses bacterial pathogen-induced activation of human neutrophils.

Authors:  M M Yurinskaya; M B Evgen'ev; O Yu Antonova; M G Vinokurov
Journal:  Dokl Biochem Biophys       Date:  2010-12-24       Impact factor: 0.788

4.  A humanized single-chain antibody against beta 3 integrin inhibits pulmonary metastasis by preferentially fragmenting activated platelets in the tumor microenvironment.

Authors:  Wei Zhang; Suying Dang; Tao Hong; Jian Tang; Jing Fan; Dawei Bu; Yanjie Sun; Zhugang Wang; Thomas Wisniewski
Journal:  Blood       Date:  2012-08-09       Impact factor: 22.113

5.  Improving the methods for isolation of monocyte and establishing macrophage cell culture in caprine model.

Authors:  Nurrul Nasruddin Shaqinah; Mazlan Mazlina; M Zamri-Saad; Hamzah Hazilawati; Sabri Jasni
Journal:  Cytotechnology       Date:  2014-12-16       Impact factor: 2.058

Review 6.  Recent Advances in Good Manufacturing Practice-Grade Generation of Dendritic Cells.

Authors:  Sarah Cunningham; Holger Hackstein
Journal:  Transfus Med Hemother       Date:  2020-10-28       Impact factor: 3.747

7.  Acute loss of renal function attenuates slow leukocyte rolling and transmigration by interfering with intracellular signaling.

Authors:  Jan Rossaint; Oliver Spelten; Nadja Kässens; Helena Mueller; Hugo K Van Aken; Kai Singbartl; Alexander Zarbock
Journal:  Kidney Int       Date:  2011-05-11       Impact factor: 10.612

8.  Cell to rodent: toxicological profiling of folate grafted thiomer enveloped nanoliposomes.

Authors:  Muhammad Farhan Sohail; Hafiz Shoaib Sarwar; Ibrahim Javed; Akhtar Nadhman; Syed Zajif Hussain; Hamid Saeed; Abida Raza; Nadeem Irfan Bukhari; Irshad Hussain; Gul Shahnaz
Journal:  Toxicol Res (Camb)       Date:  2017-07-24       Impact factor: 3.524

9.  Isolation, characterisation and phagocytic function of human macrophages from human peripheral blood.

Authors:  Lidija Gradišnik; Marko Milojević; Tomaž Velnar; Uroš Maver
Journal:  Mol Biol Rep       Date:  2020-09-02       Impact factor: 2.316

10.  Human immunodeficiency virus type 1 V1-to-V5 envelope variants from the chronic phase of infection use CCR5 and fuse more efficiently than those from early after infection.

Authors:  Behzad Etemad; Angela Fellows; Brenda Kwambana; Anupa Kamat; Yang Feng; Sandra Lee; Manish Sagar
Journal:  J Virol       Date:  2009-07-22       Impact factor: 5.103
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.