K N Qin1, R L Rosenfield. 1. Pritzker School of Medicine, University of Chicago Children's Hospital, University of Chicago, Illinois 60637-1470, USA.
Abstract
OBJECTIVE: Conversion of androstenedione to testosterone, the most potent androgen secreted by the ovary, is carried out by androgenic 17 beta-hydroxysteroid dehydrogenase (17 beta-HSD) activity. The molecular basis for this is unclear. We tested the hypothesis that type 5 17 beta-HSD (17 beta-HSD5) is responsible for testosterone formation from androstenedione in the human ovary. METHODS: We used primers specific for each type of 17 beta-HSD to identify quantitatively and directly sequence the polymerase chain reaction products of a human ovary library. RESULTS: 17 beta-HSD1, 17 beta-HSD4, and 17 beta-HSD5 were detected in the library lysate, but not 17 beta-HSD2 or 17 beta-HSD3. 17 beta-HSD5 was the predominant androgenic form of 17 beta-HSD expressed in human ovary. CONCLUSION: These data suggest that 17 beta-HSD5 may play a major role in testosterone biosynthesis by the human ovary. Further investigation of the regulation of 17 beta-HSD5 gene expression is warranted with regard to ovarian testosterone secretion in normal and abnormal states of ovarian function, such as polycystic ovary syndrome.
OBJECTIVE: Conversion of androstenedione to testosterone, the most potent androgen secreted by the ovary, is carried out by androgenic 17 beta-hydroxysteroid dehydrogenase (17 beta-HSD) activity. The molecular basis for this is unclear. We tested the hypothesis that type 5 17 beta-HSD (17 beta-HSD5) is responsible for testosterone formation from androstenedione in the human ovary. METHODS: We used primers specific for each type of 17 beta-HSD to identify quantitatively and directly sequence the polymerase chain reaction products of a human ovary library. RESULTS: 17 beta-HSD1, 17 beta-HSD4, and 17 beta-HSD5 were detected in the library lysate, but not 17 beta-HSD2 or 17 beta-HSD3. 17 beta-HSD5 was the predominant androgenic form of 17 beta-HSD expressed in human ovary. CONCLUSION: These data suggest that 17 beta-HSD5 may play a major role in testosterone biosynthesis by the human ovary. Further investigation of the regulation of 17 beta-HSD5 gene expression is warranted with regard to ovarian testosterone secretion in normal and abnormal states of ovarian function, such as polycystic ovary syndrome.
Authors: U Hoppe; P-M Holterhus; L Wünsch; D Jocham; T Drechsler; S Thiele; C Marschke; O Hiort Journal: J Mol Med (Berl) Date: 2006-03-25 Impact factor: 4.599
Authors: Kenan Qin; David A Ehrmann; Nancy Cox; Samuel Refetoff; Robert L Rosenfield Journal: J Clin Endocrinol Metab Date: 2005-11-01 Impact factor: 5.958
Authors: Evana Valenzuela Scheker; Amita Kathuria; Ashwini Esnakula; Hironobu Sasano; Yuto Yamazaki; Sergei Tevosian; Richard J Auchus; Hans K Ghayee; Gauri Dhir Journal: J Investig Med High Impact Case Rep Date: 2020 Jan-Dec