| Literature DB >> 10731150 |
R A Hoskins1, C R Nelson, B P Berman, T R Laverty, R A George, L Ciesiolka, M Naeemuddin, A D Arenson, J Durbin, R G David, P E Tabor, M R Bailey, D R DeShazo, J Catanese, A Mammoser, K Osoegawa, P J de Jong, S E Celniker, R A Gibbs, G M Rubin, S E Scherer.
Abstract
We constructed a bacterial artificial chromosome (BAC)-based physical map of chromosomes 2 and 3 of Drosophila melanogaster, which constitute 81% of the genome. Sequence tagged site (STS) content, restriction fingerprinting, and polytene chromosome in situ hybridization approaches were integrated to produce a map spanning the euchromatin. Three of five remaining gaps are in repeat-rich regions near the centromeres. A tiling path of clones spanning this map and STS maps of chromosomes X and 4 was sequenced to low coverage; the maps and tiling path sequence were used to support and verify the whole-genome sequence assembly, and tiling path BACs were used as templates in sequence finishing.Entities:
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Year: 2000 PMID： 10731150 DOI： 10.1126/science.287.5461.2271
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728