OBJECTIVE: To analyze which physiologic stimuli induce secretion of myeloid-related protein 8 (MRP8) and MRP14, two S100 proteins expressed in neutrophils and monocytes, and to determine whether serum concentrations of these proteins are reliable parameters for monitoring inflammatory activity in pauciarticular juvenile rheumatoid arthritis (JRA). METHODS: Secretion of MRP8 and MRP14 was analyzed using a coculture system of endothelial cells and monocytes. Concentrations of MRP8/MRP14 in the serum and synovial fluid of JRA patients or culture medium were determined by enzyme-linked immunosorbent assay. The expression of MRP8 and MRP14 by leukocytes in synovial tissue or fluid was investigated using immunohistochemistry. RESULTS: MRP8 and MRP14 were specifically released during interaction of activated monocytes with tumor necrosis factor-stimulated endothelial cells. Secretion was mediated via an increase in intracellular calcium levels in monocytes. In contrast, contact with resting endothelium inhibited protein kinase C-induced secretion of the proteins by monocytes. In JRA patients, MRP8 and MRP14 were strongly expressed in infiltrating neutrophils and monocytes within the inflamed joints and could be found in significantly higher concentrations in synovial fluid (mean 42,800 ng/ml) compared with serum (2,060 ng/ml). Concentrations of MRP8/MRP14 in serum correlated well with those in synovial fluid (r = 0.78) and showed a strong correlation with disease activity (r = 0.62). After intraarticular triamcinolone therapy, the serum concentrations of MRP8/MRP14 decreased significantly in therapy responders, whereas no differences were found in patients who showed no clinical benefit. CONCLUSION: MRP8 and MRP14 are specifically released during the interaction of monocytes with inflammatory activated endothelium, probably at sites of local inflammation. Their serum concentrations represent a useful marker for monitoring local inflammation in JRA.
OBJECTIVE: To analyze which physiologic stimuli induce secretion of myeloid-related protein 8 (MRP8) and MRP14, two S100 proteins expressed in neutrophils and monocytes, and to determine whether serum concentrations of these proteins are reliable parameters for monitoring inflammatory activity in pauciarticular juvenile rheumatoid arthritis (JRA). METHODS: Secretion of MRP8 and MRP14 was analyzed using a coculture system of endothelial cells and monocytes. Concentrations of MRP8/MRP14 in the serum and synovial fluid of JRA patients or culture medium were determined by enzyme-linked immunosorbent assay. The expression of MRP8 and MRP14 by leukocytes in synovial tissue or fluid was investigated using immunohistochemistry. RESULTS:MRP8 and MRP14 were specifically released during interaction of activated monocytes with tumor necrosis factor-stimulated endothelial cells. Secretion was mediated via an increase in intracellular calcium levels in monocytes. In contrast, contact with resting endothelium inhibited protein kinase C-induced secretion of the proteins by monocytes. In JRA patients, MRP8 and MRP14 were strongly expressed in infiltrating neutrophils and monocytes within the inflamed joints and could be found in significantly higher concentrations in synovial fluid (mean 42,800 ng/ml) compared with serum (2,060 ng/ml). Concentrations of MRP8/MRP14 in serum correlated well with those in synovial fluid (r = 0.78) and showed a strong correlation with disease activity (r = 0.62). After intraarticular triamcinolone therapy, the serum concentrations of MRP8/MRP14 decreased significantly in therapy responders, whereas no differences were found in patients who showed no clinical benefit. CONCLUSION:MRP8 and MRP14 are specifically released during the interaction of monocytes with inflammatory activated endothelium, probably at sites of local inflammation. Their serum concentrations represent a useful marker for monitoring local inflammation in JRA.
Authors: Caroline Soulas; Cecily Conerly; Woong-Ki Kim; Tricia H Burdo; Xavier Alvarez; Andrew A Lackner; Kenneth C Williams Journal: Am J Pathol Date: 2011-05 Impact factor: 4.307
Authors: Stephan Seeliger; Thomas Vogl; Ingo Hubert Engels; J Michael Schröder; Clemens Sorg; Cord Sunderkötter; Johannes Roth Journal: Am J Pathol Date: 2003-09 Impact factor: 4.307
Authors: Kenneth Hsu; Chantrakorn Champaiboon; Brian D Guenther; Brent S Sorenson; Ali Khammanivong; Karen F Ross; Carolyn L Geczy; Mark C Herzberg Journal: Antiinflamm Antiallergy Agents Med Chem Date: 2009-12-04
Authors: Andrei Maiseyeu; Marcus A Badgeley; Thomas Kampfrath; Georgeta Mihai; Jeffrey A Deiuliis; Cuiqing Liu; Qinghua Sun; Sampath Parthasarathy; Daniel I Simon; Kevin Croce; Sanjay Rajagopalan Journal: Arterioscler Thromb Vasc Biol Date: 2012-02-02 Impact factor: 8.311
Authors: Radha Gopal; Leticia Monin; Diana Torres; Samantha Slight; Smriti Mehra; Kyle C McKenna; Beth A Fallert Junecko; Todd A Reinhart; Jay Kolls; Renata Báez-Saldaña; Alfredo Cruz-Lagunas; Tatiana S Rodríguez-Reyna; Nathella Pavan Kumar; Phillipe Tessier; Johannes Roth; Moisés Selman; Enrique Becerril-Villanueva; Javier Baquera-Heredia; Bridgette Cumming; Victoria O Kasprowicz; Adrie J C Steyn; Subash Babu; Deepak Kaushal; Joaquín Zúñiga; Thomas Vogl; Javier Rangel-Moreno; Shabaana A Khader Journal: Am J Respir Crit Care Med Date: 2013-11-01 Impact factor: 21.405
Authors: Mikel Alberdi-Saugstrup; Susan Nielsen; Pernille Mathiessen; Claus Henrik Nielsen; Klaus Müller Journal: Clin Rheumatol Date: 2016-08-25 Impact factor: 2.980