| Literature DB >> 10728569 |
J Whyte1, A D Roberts, K A Morley, R J Sharp, P D Marsh.
Abstract
A human-derived monocytic cell line (U937) was induced to phagocytose Mycobacterium phlei by the addition of phorbol myristate acetate (PMA) to the culture medium for 50-60 h. Cells not treated with PMA were unable to phagocytose M. phlei. Magnetic beads enabled a rapid and highly efficient separation of phagocytosed and free bacteria to be achieved, an approach which is particularly useful if colony plating is used to enumerate bacterial survival within phagocytic cells. Fluorescence-activated cell sorting (FACS) analysis showed that 98% of U937 cells contained viable bacteria after 3 h.Entities:
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Year: 2000 PMID： 10728569 DOI： 10.1046/j.1472-765x.2000.00701.x
Source DB: PubMed Journal: Lett Appl Microbiol ISSN： 0266-8254 Impact factor: 2.858