Comparison of drought tolerance in nitrogen-fixing and inorganic nitrogen-grown common beans.

In this work, we evaluated how the use of alternative N sources affects drought-stress tolerance in common beans. To this end, plants were cultivated employing either N(2) fixation or two levels of inorganic nitrogen: 1 mM NH(4)NO(3) (limiting) or 10 mM NH(4)NO(3) (sufficient). Drought was imposed by withholding watering at 30 days after planting (DAP) - coinciding with flowering. At 20 DAP, growth and N content were significantly higher in NH(4)NO(3)-sufficient plants than in N(2)-fixing and NH(4)NO(3)-limited beans. At later times, only N(2)-fixing and NH(4)NO(3)-sufficient plants continued assimilating N and growing, with the NH(4)NO(3)-sufficient plants being consistently bigger. After 10 days of stress (40 DAP), desiccation was evident, but only NH(4)NO(3)-sufficient plants suffered drought-induced senescence. After 20 days of stress (50 DAP), N content increased in NH(4)NO(3)-sufficient but not in N(2)-fixing beans, despite the latter's lesser state of wilt. Pod dry weight dropped 43% in NH(4)NO(3)-sufficient beans with respect to well-watered plants, while remaining constant in N(2)-fixing beans. Under drought conditions, the number of pods limited pod yield regardless of the nitrogen source used; nevertheless, the translocation of soluble matter to pods continued in both NH(4)NO(3)-sufficient and N(2)-fixing beans. We conclude that common beans grown under conditions of N(2) fixation were more drought tolerant than those provided with sufficient levels of NH(4)NO(3). The most stress-sensitive traits in these plants were the incorporation of N into their shoots and the number of pods remaining on them.