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Literature DB >> 10722637

Stable expression of a new chimeric fluorescent reporter in the human malaria parasite Plasmodium falciparum.

M Kadekoppala¹, K Kline, T Akompong, K Haldar.

Abstract

Stable transfection of a new, chimeric reporter in the human malaria parasite Plasmodium falciparum confers green fluorescence and methotrexate resistance that can be quantitated by Western blotting and flow cytometry. This provides a sensitive, live reporter for exploitation of genomic and high-throughput assays for the identification of new pathogenic determinants.

Entities: Chemical Disease Species

Mesh：

Substances：

Year: 2000 PMID： 10722637 PMCID： PMC97421 DOI： 10.1128/IAI.68.4.2328-2332.2000

Source DB: PubMed Journal: Infect Immun ISSN： 0019-9567 Impact factor: 3.441

17 in total

1. Green fluorescent protein as a marker in Plasmodium berghei transformation.

Authors: A A Sultan; V Thathy; V Nussenzweig; R Ménard
Journal: Infect Immun Date: 1999-05 Impact factor: 3.441

2. Characterization of promoters and stable transfection by homologous and nonhomologous recombination in Plasmodium falciparum.

Authors: B S Crabb; A F Cowman
Journal: Proc Natl Acad Sci U S A Date: 1996-07-09 Impact factor: 11.205

3. Stable expression of green fluorescent protein in blood and mosquito stages of Plasmodium berghei.

Authors: T F de Koning-Ward; A W Thomas; A P Waters; C J Janse
Journal: Mol Biochem Parasitol Date: 1998-11-30 Impact factor: 1.759

4. Transformation of Plasmodium falciparum malaria parasites by homologous integration of plasmids that confer resistance to pyrimethamine.

Authors: Y Wu; L A Kirkman; T E Wellems
Journal: Proc Natl Acad Sci U S A Date: 1996-02-06 Impact factor: 11.205

5. Transformation with human dihydrofolate reductase renders malaria parasites insensitive to WR99210 but does not affect the intrinsic activity of proguanil.

Authors: D A Fidock; T E Wellems
Journal: Proc Natl Acad Sci U S A Date: 1997-09-30 Impact factor: 11.205

6. Stable transgene expression in Plasmodium falciparum.

Authors: B S Crabb; T Triglia; J G Waterkeyn; A F Cowman
Journal: Mol Biochem Parasitol Date: 1997-12-01 Impact factor: 1.759

7. A set of independent selectable markers for transfection of the human malaria parasite Plasmodium falciparum.

Authors: C B Mamoun; I Y Gluzman; S Goyard; S M Beverley; D E Goldberg
Journal: Proc Natl Acad Sci U S A Date: 1999-07-20 Impact factor: 11.205

8. Stable transfection of malaria parasite blood stages.

Authors: M R van Dijk; A P Waters; C J Janse
Journal: Science Date: 1995-06-02 Impact factor: 47.728

9. Transfection of the malaria parasite and expression of firefly luciferase.

Authors: R Goonewardene; J Daily; D Kaslow; T J Sullivan; P Duffy; R Carter; K Mendis; D Wirth
Journal: Proc Natl Acad Sci U S A Date: 1993-06-01 Impact factor: 11.205

10. Transfection of Plasmodium falciparum within human red blood cells.

Authors: Y Wu; C D Sifri; H H Lei; X Z Su; T E Wellems
Journal: Proc Natl Acad Sci U S A Date: 1995-02-14 Impact factor: 11.205

16 in total

1. Cooperative domains define a unique host cell-targeting signal in Plasmodium falciparum-infected erythrocytes.

Authors: Carlos Lopez-Estraño; Souvik Bhattacharjee; Travis Harrison; Kasturi Haldar
Journal: Proc Natl Acad Sci U S A Date: 2003-09-26 Impact factor: 11.205

2. Red Blood Cell Invasion by the Malaria Parasite Is Coordinated by the PfAP2-I Transcription Factor.

Authors: Joana Mendonca Santos; Gabrielle Josling; Philipp Ross; Preeti Joshi; Lindsey Orchard; Tracey Campbell; Ariel Schieler; Ileana M Cristea; Manuel Llinás
Journal: Cell Host Microbe Date: 2017-06-14 Impact factor: 21.023

3. Plasmodium falciparum: development of a transgenic line for screening antimalarials using firefly luciferase as the reporter.

Authors: Long Cui; Jun Miao; Jiaxu Wang; Qianjun Li; Liwang Cui
Journal: Exp Parasitol Date: 2008-05-29 Impact factor: 2.011

Stable expression of a new chimeric fluorescent reporter in the human malaria parasite Plasmodium falciparum.

1. Green fluorescent protein as a marker in Plasmodium berghei transformation.

2. Characterization of promoters and stable transfection by homologous and nonhomologous recombination in Plasmodium falciparum.

3. Stable expression of green fluorescent protein in blood and mosquito stages of Plasmodium berghei.

4. Transformation of Plasmodium falciparum malaria parasites by homologous integration of plasmids that confer resistance to pyrimethamine.

5. Transformation with human dihydrofolate reductase renders malaria parasites insensitive to WR99210 but does not affect the intrinsic activity of proguanil.

6. Stable transgene expression in Plasmodium falciparum.

7. A set of independent selectable markers for transfection of the human malaria parasite Plasmodium falciparum.

8. Stable transfection of malaria parasite blood stages.

9. Transfection of the malaria parasite and expression of firefly luciferase.

10. Transfection of Plasmodium falciparum within human red blood cells.

1. Cooperative domains define a unique host cell-targeting signal in Plasmodium falciparum-infected erythrocytes.

2. Red Blood Cell Invasion by the Malaria Parasite Is Coordinated by the PfAP2-I Transcription Factor.

3. Plasmodium falciparum: development of a transgenic line for screening antimalarials using firefly luciferase as the reporter.

4. Validating a firefly luciferase-based high-throughput screening assay for antimalarial drug discovery.

Review 5. Methodology and application of flow cytometry for investigation of human malaria parasites.

6. Branched tricarboxylic acid metabolism in Plasmodium falciparum.

7. Simple and inexpensive fluorescence-based technique for high-throughput antimalarial drug screening.

8. Sex- and stage-specific reporter gene expression in Plasmodium falciparum.

9. High-content live cell imaging with RNA probes: advancements in high-throughput antimalarial drug discovery.

10. piggyBac is an effective tool for functional analysis of the Plasmodium falciparum genome.