| Literature DB >> 10722071 |
E G de Jalón1, M Josa, M A Campanero, S Santoyo, P Ygartua.
Abstract
A direct, simple and rapid high-performance liquid chromatographic method has been developed for the determination of ketoprofen with ibuprofen as internal standard. Samples were chromatographed on a 5 microm Kromasil 100 C18 column. The mobile phase was a mixture of acetonitrile-0.01 M KH2PO4 adjusted to pH 1.5 with orthophosphoric acid 85% (60:40, v/v). Detection was at 260 nm and the run time was 10 min. The detector response was found to be linear in the concentration range 0.02 to 40 microg/ml. This HPLC assay has been applied to measure the "in vitro" percutaneous penetration of ketoprofen through rat skin.Entities:
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Year: 2000 PMID： 10722071 DOI： 10.1016/s0021-9673(99)00995-4
Source DB: PubMed Journal: J Chromatogr A ISSN： 0021-9673 Impact factor: 4.759