BACKGROUND: Mast cells are resident connective tissue cells able to secrete numerous inflammatory mediators in response to tissue aggression and might be implicated in the fibrotic processes. OBJECTIVES: To study the effects of mast cell products on fibroblast activity in connective tissues. METHODS: Mast cell extract was prepared by sonication of pure mast cell preparations obtained by peritoneal lavage of rats and added to the culture medium of fibroblast-populated collagen lattices. RESULTS: Mast cell extract was able to decrease the contraction of the collagen lattices, to stimulate total protein and collagen synthesis, and to increase the expression and activation of gelatinase A/MMP-2. CONCLUSION: These data are consistent with the hypothesis that mast cells in connective tissue may be responsible for fibroblast activation at the early phases of tissue repair and fibrosis.
BACKGROUND: Mast cells are resident connective tissue cells able to secrete numerous inflammatory mediators in response to tissue aggression and might be implicated in the fibrotic processes. OBJECTIVES: To study the effects of mast cell products on fibroblast activity in connective tissues. METHODS: Mast cell extract was prepared by sonication of pure mast cell preparations obtained by peritoneal lavage of rats and added to the culture medium of fibroblast-populated collagen lattices. RESULTS: Mast cell extract was able to decrease the contraction of the collagen lattices, to stimulate total protein and collagen synthesis, and to increase the expression and activation of gelatinase A/MMP-2. CONCLUSION: These data are consistent with the hypothesis that mast cells in connective tissue may be responsible for fibroblast activation at the early phases of tissue repair and fibrosis.
Authors: Scott M Bolton; Amir F Kagalwalla; Nicoleta C Arva; Ming-Yu Wang; Katie Amsden; Hector Melin-Aldana; Evan S Dellon; Paul J Bryce; Barry K Wershil; Joshua B Wechsler Journal: Am J Gastroenterol Date: 2020-02 Impact factor: 12.045
Authors: J Pingel; J Wienecke; M Kongsgaard; H Behzad; T Abraham; H Langberg; A Scott Journal: Scand J Med Sci Sports Date: 2013-07-25 Impact factor: 4.221
Authors: Craig C Reed; Robert M Genta; Bradford A Youngblood; Joshua B Wechsler; Evan S Dellon Journal: Clin Gastroenterol Hepatol Date: 2020-08-12 Impact factor: 13.576
Authors: Águida Cristina Gomes Henriques; Roseana de Almeida Freitas; Bruno Cunha Pires; Clarissa Araújo Gurgel; Jean Nunes Dos Santos Journal: An Bras Dermatol Date: 2016 Sep-Oct Impact factor: 1.896