E Molnar-Gabor1, E Endreffy, A Rozsasi. 1. Department of Otorhinolaryngology, Albert Szent-Györgyi Medical University, Szeged, Hungary.
Abstract
OBJECTIVES/HYPOTHESIS: Genetic etiology is suspected in the development of nasal polyposis on the basis of familial aggregation. This study investigated whether there is an association between HLA-DRB1, -DQA1, and -DQB1 alleles and developing nasal polyposis. STUDY DESIGN: Data from 50 polypectomized patients were compared with data from 50 healthy randomly selected controls. Polyp score, possible asthma, aspirin sensitivity, and ASA triad were also recorded. METHODS: Genotyping of HLA-DRB1 alleles was carried out with the Dynal RELI SSO HLA-DRB, a direct DNA probe test that utilizes a polymerase chain reaction (PCR) and nucleic acid hybridization for the differentiation of 70 HLA-DRB alleles and 9 supertypes. For DQA1* genotyping PCR-RFLP (restriction fragment length polymorphism) was used, differentiating eight alleles. The DQB1* typing was carried out using a INNO-LiPA DQB PCR-reverse hybridization kit, allowing the discrimination of 30 alleles. RESULTS: People carrying the HLA-DR7-DQA1*0201, and -DQB1*0202 haplotype were found to have a two to three times higher odds ratios (ORs) for developing the disease, compared with controls. Patients with ASA triad carried the above-mentioned DR7 allele with the linked alleles significantly more often (P < .001). Subjects carrying HLA-DR5 allele and the linked alleles had lower odds ratio values. CONCLUSION: These results underline that allergy is not conditional for the formation of nasal polyps as thought before. Nasal polyposis associated with asthma and aspirin sensitivity is probably a unique form of nasal polyps. The authors plan further investigations in this field.
OBJECTIVES/HYPOTHESIS: Genetic etiology is suspected in the development of nasal polyposis on the basis of familial aggregation. This study investigated whether there is an association between HLA-DRB1, -DQA1, and -DQB1 alleles and developing nasal polyposis. STUDY DESIGN: Data from 50 polypectomized patients were compared with data from 50 healthy randomly selected controls. Polyp score, possible asthma, aspirin sensitivity, and ASA triad were also recorded. METHODS: Genotyping of HLA-DRB1 alleles was carried out with the Dynal RELI SSO HLA-DRB, a direct DNA probe test that utilizes a polymerase chain reaction (PCR) and nucleic acid hybridization for the differentiation of 70 HLA-DRB alleles and 9 supertypes. For DQA1* genotyping PCR-RFLP (restriction fragment length polymorphism) was used, differentiating eight alleles. The DQB1* typing was carried out using a INNO-LiPA DQB PCR-reverse hybridization kit, allowing the discrimination of 30 alleles. RESULTS:People carrying the HLA-DR7-DQA1*0201, and -DQB1*0202 haplotype were found to have a two to three times higher odds ratios (ORs) for developing the disease, compared with controls. Patients with ASA triad carried the above-mentioned DR7 allele with the linked alleles significantly more often (P < .001). Subjects carrying HLA-DR5 allele and the linked alleles had lower odds ratio values. CONCLUSION: These results underline that allergy is not conditional for the formation of nasal polyps as thought before. Nasal polyposis associated with asthma and aspirin sensitivity is probably a unique form of nasal polyps. The authors plan further investigations in this field.
Authors: Heather K Lehman; Michelle R Simpson-Abelson; Thomas F Conway; Raymond J Kelleher; Joel M Bernstein; Richard B Bankert Journal: J Assoc Res Otolaryngol Date: 2012-02-04
Authors: Yohan Bossé; François Bacot; Alexandre Montpetit; Johan Rung; Hui-Qi Qu; James C Engert; Constantin Polychronakos; Thomas J Hudson; Philippe Froguel; Robert Sladek; Martin Desrosiers Journal: Hum Genet Date: 2009-01-29 Impact factor: 4.132
Authors: Joy Hsu; Pedro C Avila; Robert C Kern; M Geoffrey Hayes; Robert P Schleimer; Jayant M Pinto Journal: J Allergy Clin Immunol Date: 2013-04 Impact factor: 10.793
Authors: J Lasky-Su; B E Himes; B A Raby; B J Klanderman; J Senter Sylvia; C Lange; E Melen; F D Martinez; E Israel; J Gauderman; F Gilliland; P Sleiman; H Hakonarson; J C Celedón; M Soto-Quiros; L Avila; J J Lima; C G Irvin; S P Peters; H Boushey; V M Chinchilli; D Mauger; K Tantisira; S T Weiss Journal: Clin Exp Allergy Date: 2012-12 Impact factor: 5.018