Literature DB >> 10715017

Purification and properties of ornithine racemase from Clostridium sticklandii.

H P Chen1, C F Lin, Y J Lee, S S Tsay, S H Wu.   

Abstract

Ornithine racemase has been purified to homogeneity from Clostridium sticklandii, as shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. This is the first racemase known to be highly specific to ornithine. This PLP-dependent enzyme has an M(r) of 92, 000, with a K(m) for L-ornithine of 0.77 +/- 0.05 mM and a k(cat) of 980 +/- 20 s(-1).

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Year:  2000        PMID： 10715017      PMCID： PMC101933          DOI： 10.1128/JB.182.7.2052-2054.2000

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  8 in total

1.  Ornithine degradation in Clostridium sticklandii; pyridoxal phosphate and coenzyme A dependent thiolytic cleavage of 2-amino-4-ketopentanoate to alanine and acetyl coenzyme A.

Authors:  I M Jeng; R Somack; H A Barker
Journal:  Biochemistry       Date:  1974-07-02       Impact factor: 3.162

2.  Purification and properties of a pyridoxal phosphate and coenzyme B 12 dependent D- -ornithine 5,4-aminomutase.

Authors:  R Somack; R N Costilow
Journal:  Biochemistry       Date:  1973-07-03       Impact factor: 3.162

3.  2,4-diaminopentanoic acid C 4 dehydrogenase. Purification and properties of the protein.

Authors:  R Somack; R N Costilow
Journal:  J Biol Chem       Date:  1973-01-25       Impact factor: 5.157

4.  Arginine racemase of Pseudomonas graveolens. I. Purification, crystallization, and properties.

Authors:  T Yorifuji; K Ogata
Journal:  J Biol Chem       Date:  1971-08-25       Impact factor: 5.157

5.  Preparation and characterization of 2,4-diaminovaleric acid: an intermediate in the anaerobic oxidation of ornithine.

Authors:  R L Somack; D H Bing; R N Costilow
Journal:  Anal Biochem       Date:  1971-05       Impact factor: 3.365

Review 6.  Amino acid degradation by anaerobic bacteria.

Authors:  H A Barker
Journal:  Annu Rev Biochem       Date:  1981       Impact factor: 23.643

7.  Reaction mechanism and structure of the active site of proline racemase.

Authors:  G Rudnick; R H Abeles
Journal:  Biochemistry       Date:  1975-10-07       Impact factor: 3.162

8.  A new amino acid racemase with threonine alpha-epimerase activity from Pseudomonas putida: purification and characterization.

Authors:  Y H Lim; K Yokoigawa; N Esaki; K Soda
Journal:  J Bacteriol       Date:  1993-07       Impact factor: 3.490
  8 in total
  4 in total

1.  Enzymatic tailoring of ornithine in the biosynthesis of the Rhizobium cyclic trihydroxamate siderophore vicibactin.

Authors:  John R Heemstra; Christopher T Walsh; Elizabeth S Sattely
Journal:  J Am Chem Soc       Date:  2009-10-28       Impact factor: 15.419

2.  Identification, purification, and characterization of a novel amino acid racemase, isoleucine 2-epimerase, from Lactobacillus species.

Authors:  Yuta Mutaguchi; Taketo Ohmori; Taisuke Wakamatsu; Katsumi Doi; Toshihisa Ohshima
Journal:  J Bacteriol       Date:  2013-09-13       Impact factor: 3.490

3.  A conserved gene cluster rules anaerobic oxidative degradation of L-ornithine.

Authors:  Nuria Fonknechten; Alain Perret; Nadia Perchat; Sabine Tricot; Christophe Lechaplais; David Vallenet; Carine Vergne; Anne Zaparucha; Denis Le Paslier; Jean Weissenbach; Marcel Salanoubat
Journal:  J Bacteriol       Date:  2009-02-27       Impact factor: 3.490

Review 4.  Amino acid catabolism-directed biofuel production in Clostridium sticklandii: An insight into model-driven systems engineering.

Authors:  C Sangavai; P Chellapandi
Journal:  Biotechnol Rep (Amst)       Date:  2017-11-08
  4 in total

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