D Yao1, D Jiang, X Wu. 1. Affiliated Hospital, Nantong Medical College, Nantong 226001, China.
Abstract
OBJECTIVE: To explore the value of gama-glutamyl transferase (GGT) abnormal expression in monitoring carcinogenesis of hepatocytes. METHODS: The kinetic alterations of rat (SD) liver pathology, enzymatic histochemistry, total RNA levels, different molecular form GGT and its isoenzymes were investigated on experimental hepatomas inducing with 0.05% 2-fluoenylacetamide (2-FAA). RESULTS: The GGTs of livers were overexpressed during canceration, liver GGT specific activities (U/g) including soluble and membrane-combined GGT activities of each group in experiment rats were significantly higher (q=4.90, P<0.01) than those of control group. The total RNA concentration was highly positively correlated with liver GGT specific activities or serum GGT activities (r=0.90, r=0.79, P<0.01). The mixed isoenzyme patterns of control rats' and embryo rats' GGT were found in sera and livers of experimental rats, the carcino-embryonic GGT bands can be detected at early stages of rat hepatocyte canceration. CONCLUSIONS: The present data suggest that GGT is a sensitive enzyme for carcinogenesis of hepatocytes, the analysis of carcino-embryonic GGT is a useful marker for early diagnosis of rat hepatoma.
OBJECTIVE: To explore the value of gama-glutamyl transferase (GGT) abnormal expression in monitoring carcinogenesis of hepatocytes. METHODS: The kinetic alterations of rat (SD) liver pathology, enzymatic histochemistry, total RNA levels, different molecular form GGT and its isoenzymes were investigated on experimental hepatomas inducing with 0.05% 2-fluoenylacetamide (2-FAA). RESULTS: The GGTs of livers were overexpressed during canceration, liver GGT specific activities (U/g) including soluble and membrane-combined GGT activities of each group in experiment rats were significantly higher (q=4.90, P<0.01) than those of control group. The total RNA concentration was highly positively correlated with liver GGT specific activities or serum GGT activities (r=0.90, r=0.79, P<0.01). The mixed isoenzyme patterns of control rats' and embryo rats' GGT were found in sera and livers of experimental rats, the carcino-embryonic GGT bands can be detected at early stages of rat hepatocyte canceration. CONCLUSIONS: The present data suggest that GGT is a sensitive enzyme for carcinogenesis of hepatocytes, the analysis of carcino-embryonic GGT is a useful marker for early diagnosis of rathepatoma.