J Cheng1, Y Zhong. 1. Gene Therapy Research Center, Institute of Infectious Diseases, the 302 Hospital of PLA, Beijing 100039, China.
Abstract
OBJECTIVE: To clone the human genomic DNA of augmenter of liver regeneration (ALR) and specify the intron-exon structure. METHODS: Using human ALR cDNA sequence as a reference and BLAST path as a nucleotide homology search tool, GenBank has been searched for ALR homologous genomic DNA sequence. The intron-exon sequences were defined by the Breathnath-Chambon rule. RESULTS: The coding sequence of human ALR consists of 3 exons, and is similar to murine ALR genomic DNA structure. The human ALR genomic DNA is 1813 nt long and codes a protein of 125 amino acid residues. CONCLUSION: Human genomic DNA of ALR consists of 3 exons and 2 introns.
OBJECTIVE: To clone the human genomic DNA of augmenter of liver regeneration (ALR) and specify the intron-exon structure. METHODS: Using humanALR cDNA sequence as a reference and BLAST path as a nucleotide homology search tool, GenBank has been searched for ALR homologous genomic DNA sequence. The intron-exon sequences were defined by the Breathnath-Chambon rule. RESULTS: The coding sequence of humanALR consists of 3 exons, and is similar to murineALR genomic DNA structure. The humanALR genomic DNA is 1813 nt long and codes a protein of 125 amino acid residues. CONCLUSION:Human genomic DNA of ALR consists of 3 exons and 2 introns.