OBJECTIVE: The 13C-urea breath test (13C-UBT) is a safe, noninvasive, and accurate test for the detection of Helicobacter pylori (H. pylori) infection in adults. The aim of this study was to evaluate sensitivity and specificity of 13C-UBT in children using different types of test meal, doses of 13C-urea and breath sampling intervals. As yet, a validated, standardized 13C-UBT protocol for children has not been formulated. METHODS: 13C-UBT was performed in 115 children and repeated within 3 days, modifying the test meal or the dose of 13C-urea. H. pylori status was assessed by histology and rapid urease test. 13C-UBT was performed using 100 mg or 50 mg of 13C-urea and a fatty test meal (100 FA; 50 FA), 50 mg of 13C-urea, and a carbohydrate test meal (50 CA). Breath samples were collected every 10 min for 60 min. RESULTS: The 13C-UBT in children was highly sensitive and specific with all three protocols used. The best combination of sensitivity (97.92%) and specificity (97.96%) was obtained with Protocol 50 FA at 30 min with a cut-off of 3.5 per mil. CONCLUSIONS: The 13C-UBT is an accurate test for the detection of H. pylori infection also in children. Administration of 50 mg of 13C-urea, a fatty test meal, and breath sampling at 30 min appears to be the most convenient protocol.
OBJECTIVE: The 13C-urea breath test (13C-UBT) is a safe, noninvasive, and accurate test for the detection of Helicobacter pylori (H. pylori) infection in adults. The aim of this study was to evaluate sensitivity and specificity of 13C-UBT in children using different types of test meal, doses of 13C-urea and breath sampling intervals. As yet, a validated, standardized 13C-UBT protocol for children has not been formulated. METHODS:13C-UBT was performed in 115 children and repeated within 3 days, modifying the test meal or the dose of 13C-urea. H. pylori status was assessed by histology and rapid urease test. 13C-UBT was performed using 100 mg or 50 mg of 13C-urea and a fatty test meal (100 FA; 50 FA), 50 mg of 13C-urea, and a carbohydrate test meal (50 CA). Breath samples were collected every 10 min for 60 min. RESULTS: The 13C-UBT in children was highly sensitive and specific with all three protocols used. The best combination of sensitivity (97.92%) and specificity (97.96%) was obtained with Protocol 50 FA at 30 min with a cut-off of 3.5 per mil. CONCLUSIONS: The 13C-UBT is an accurate test for the detection of H. pyloriinfection also in children. Administration of 50 mg of 13C-urea, a fatty test meal, and breath sampling at 30 min appears to be the most convenient protocol.
Authors: Luciana de Carvalho Costa Cardinali; Gifone Aguiar Rocha; Andreia Maria Camargos Rocha; Sílvia Beleza de Moura; Taciana de Figueiredo Soares; Ana Maria Braz Esteves; Ana Margarida Miguel Ferreira Nogueira; Mônica Maria Demas Alvares Cabral; Anfrisina Sales Teles de Carvalho; Paulo Bitencourt; Alexandre Ferreira; Dulciene Maria Magalhães Queiroz Journal: J Clin Microbiol Date: 2003-07 Impact factor: 5.948
Authors: Dulciene Maria Magalhães Queiroz; Mayuko Saito; Gifone Aguiar Rocha; Andreia Maria Camargos Rocha; Fabrício Freire Melo; William Checkley; Lúcia Libanez Bessa C Braga; Igor Simões Silva; Robert H Gilman; Jean E Crabtree Journal: J Clin Microbiol Date: 2013-09-04 Impact factor: 5.948
Authors: Sonia Ahmed; Gail R Goldberg; Rubhana Raqib; Swapan Kumar Roy; Shahidul Haque; Vickie S Braithwaite; John M Pettifor; Ann Prentice Journal: Bone Date: 2020-04-07 Impact factor: 4.398