A Niinuma1. 1. Department of Endodontics, Nihon University School of Dentistry at Matsudo, Chiba, Japan. atsukon@mascat.nihon-u.ac.jp
Abstract
AIM: The aim of this study was to evaluate a newly developed resin (MTYA1-Ca) for direct pulp capping. METHODOLOGY: The powder of MTY1-Ca is composed of 89.0% microfiller, 10.0% calcium hydroxide and 1.0% benzoyl peroxide and was mixed with liquid (67.5% triethyleneglycol dimethacrylate, 30.0% glyceryl methacrylate, 1.0% o-methacryloyl tyrosine amide, 1.0% dimethylaminoethylmethacrylate, and 0.5% camphorquinone). The shear bond, diametral tensile, bending and compressive strengths were measured. The alkaline activity of the elute dissolved from MTYA1-Ca was calculated. Cell viability by MTT assay and alkaline phosphatase (ALPase) activity were evaluated from dental pulp fibroblast reaction to the eluate dissolved from MTYA1-Ca. Histopathological studies of the response to exposed dental pulp of beagle dogs were completed with Dycal as a control. RESULTS: The physical properties of MTYA1-Ca were significantly superior to those of Dycal. It was impossible to measure these properties with Dycal because of poor physical properties. Both MTYA1-Ca and Dycal maintained high levels of alkaline activity (pH 10.96-12.20) over the 168-h duration of the study. Cell viability by MTT assay in the intact eluate of MTYA1-Ca was significantly higher than that of Dycal, whilst ALPase showed no difference between MTYA1-Ca and Dycal. A dentine bridge formed more slowly under MTYA1-Ca than under Dycal, but similar amounts had formed at 90 days. CONCLUSIONS: MTYA1-Ca has the potential to be used as a direct pulp capping material.
AIM: The aim of this study was to evaluate a newly developed resin (MTYA1-Ca) for direct pulp capping. METHODOLOGY: The powder of MTY1-Ca is composed of 89.0% microfiller, 10.0% calcium hydroxide and 1.0% benzoyl peroxide and was mixed with liquid (67.5% triethyleneglycol dimethacrylate, 30.0% glyceryl methacrylate, 1.0% o-methacryloyl tyrosine amide, 1.0% dimethylaminoethylmethacrylate, and 0.5% camphorquinone). The shear bond, diametral tensile, bending and compressive strengths were measured. The alkaline activity of the elute dissolved from MTYA1-Ca was calculated. Cell viability by MTT assay and alkaline phosphatase (ALPase) activity were evaluated from dental pulp fibroblast reaction to the eluate dissolved from MTYA1-Ca. Histopathological studies of the response to exposed dental pulp of beagle dogs were completed with Dycal as a control. RESULTS: The physical properties of MTYA1-Ca were significantly superior to those of Dycal. It was impossible to measure these properties with Dycal because of poor physical properties. Both MTYA1-Ca and Dycal maintained high levels of alkaline activity (pH 10.96-12.20) over the 168-h duration of the study. Cell viability by MTT assay in the intact eluate of MTYA1-Ca was significantly higher than that of Dycal, whilst ALPase showed no difference between MTYA1-Ca and Dycal. A dentine bridge formed more slowly under MTYA1-Ca than under Dycal, but similar amounts had formed at 90 days. CONCLUSIONS: MTYA1-Ca has the potential to be used as a direct pulp capping material.
Authors: Lúcio P G Chicarelli; Mariana B F Webber; João P A Amorim; Ana L C A Rangel; Veridiana Camilotti; Mario A C Sinhoreti; Marcio J Mendonça Journal: Eur J Dent Date: 2020-09-08