Literature DB >> 10708399

A validated quantitative colorimetric assay for gentamicin.

P Frutos1, S Torrado, M E Perez-Lorenzo, G Frutos.   

Abstract

A colorimetric procedure was developed for the quantification of gentamicin. The method was based on the ninhydrin reaction with primary and secondary amines present in the gentamicin. This reaction produces a purple colour. The effects of several factors including pH, ninhydrin concentration and reaction time were investigated to optimize the assay method. Using the assay protocol, the absorption of the gentamicin-ninhydrin mixtures at 400 nm had a linear relationship with the gentamicin concentration ranging from 30 to 120 microg/ml. The colorimetric gentamicin assay reported herein is of great practical value because it is reproducible, sensitive, simple and extremely inexpensive.

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Year:  2000        PMID： 10708399     DOI： 10.1016/s0731-7085(99)00192-2

Source DB:  PubMed          Journal:  J Pharm Biomed Anal        ISSN： 0731-7085            Impact factor:   3.935


  7 in total

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  7 in total

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