Literature DB >> 10705446

Induction and repression of a Streptomyces lividans chitinase gene promoter in response to various carbon sources.

K Miyashita1, T Fujii, A Saito.   

Abstract

Induction and repression of a gene for chitinase (chiA) in Streptomyces lividans was investigated using a catechol 2,3-dioxygenase gene (xylE) as the reporter gene. Of various substrates examined, expression of the promoter (PchiA) was observed after a delay when colloidal chitin or small chitin-oligosaccharides were added to the medium. N-acetylglucosamine completely repressed the chiA promoter. The duration of the delay in expression of PchiA differed with the inducer used, with chitobiose inducing the activity most rapidly. The minimum concentration of chitobiose needed for induction was 1 microM. It appears, therefore, that an efficient inducer of the gene for chitinase in S. lividans is chitobiose.

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Year:  2000        PMID： 10705446     DOI： 10.1271/bbb.64.39

Source DB:  PubMed          Journal:  Biosci Biotechnol Biochem        ISSN： 0916-8451            Impact factor:   2.043


  8 in total

1.  The dasABC gene cluster, adjacent to dasR, encodes a novel ABC transporter for the uptake of N,N'-diacetylchitobiose in Streptomyces coelicolor A3(2).

Authors:  Akihiro Saito; Tomonori Shinya; Katsushiro Miyamoto; Tomofumi Yokoyama; Hanae Kaku; Eiichi Minami; Naoto Shibuya; Hiroshi Tsujibo; Yoshiho Nagata; Akikazu Ando; Takeshi Fujii; Kiyotaka Miyashita
Journal:  Appl Environ Microbiol       Date:  2007-03-09       Impact factor: 4.792

2.  Chitin and Chitosan: Production and Application of Versatile Biomedical Nanomaterials.

Authors:  Daniel Elieh-Ali-Komi; Michael R Hamblin
Journal:  Int J Adv Res (Indore)       Date:  2016-03-01

3.  Cloning of a two-component regulatory system probably involved in the regulation of chitinase in Streptomyces coelicolor A3(2).

Authors:  J Kormanec; B Sevcíková; D Homérová
Journal:  Folia Microbiol (Praha)       Date:  2000       Impact factor: 2.099

4.  Uptake of N,N'-diacetylchitobiose [(GlcNAc)2] via the phosphotransferase system is essential for chitinase production by Serratia marcescens 2170.

Authors:  Taku Uchiyama; Ryousuke Kaneko; Junko Yamaguchi; Akane Inoue; Takahiro Yanagida; Naoki Nikaidou; Miguel Regue; Takeshi Watanabe
Journal:  J Bacteriol       Date:  2003-03       Impact factor: 3.490

5.  Response regulator ChiR regulates expression of chitinase gene, chiC, in Streptomyces coelicolor.

Authors:  D Homerová; R Knirschová; J Kormanec
Journal:  Folia Microbiol (Praha)       Date:  2002       Impact factor: 2.099

6.  Bacterial chitin degradation-mechanisms and ecophysiological strategies.

Authors:  Sara Beier; Stefan Bertilsson
Journal:  Front Microbiol       Date:  2013-06-14       Impact factor: 5.640

7.  Bioinformatic identification of novel regulatory DNA sequence motifs in Streptomyces coelicolor.

Authors:  David J Studholme; Stephen D Bentley; Jan Kormanec
Journal:  BMC Microbiol       Date:  2004-04-08       Impact factor: 3.605

8.  NgcESco Acts as a Lower-Affinity Binding Protein of an ABC Transporter for the Uptake of N,N'-Diacetylchitobiose in Streptomyces coelicolor A3(2).

Authors:  Chiharu Iinuma; Akihiro Saito; Takayuki Ohnuma; Elodie Tenconi; Adeline Rosu; Séverine Colson; Yuuki Mizutani; Feng Liu; Magdalena Świątek-Połatyńska; Gilles P van Wezel; Sébastien Rigali; Takeshi Fujii; Kiyotaka Miyashita
Journal:  Microbes Environ       Date:  2018-08-07       Impact factor: 2.912
  8 in total

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