Simultaneous spectrophotometric determination of rutin, quercetin and ascorbic acid in drugs using a Kalman Filter approach.

A UV-spectrophotometric analysis of severely overlapped spectra yielded by anti-capillary fragility pharmaceutical materials, viz. rutin, quercetin and ascorbic acid, in authentic mixtures and in tablets or soft elastic capsules was proposed. The method was based on Kalman Filter calibration of either orthogonal experimental design, i.e. standard solutions containing only one component at a time, or non-orthogonal experimental design, i.e. standard solutions containing more than one component. This calibration was followed by quantitative determinations of two- and three-component mixtures in synthetic mixtures or in oral dosages within the concentration range 2-10 microg ml(-1). A statistical analysis of the results was reported.