Literature DB >> 10701835

Preparation and characterization of polyclonal antibodies against human chaperonin 10.

M J Somodevilla-Torres1, N C Hillyard, H Morton, D Alewood, J A Halliday, P F Alewood, D A Vesey, M D Walsh, A C Cavanagh.   

Abstract

Early pregnancy factor (EPF) has been identified as an extracellular homologue of chaperonin 10 (Cpn10), a heat shock protein that functions within the cell as a molecular chaperone. Here, we report the production of polyclonal antibodies directed against several different regions of the human Cpn10 molecule and their application to specific protein quantitation and localization techniques. These antibodies will be valuable tools in further studies to elucidate the mechanisms underlying the differential spatial and temporal localization of EPF and Cpn10 and in studies to elucidate structure and function.

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Year:  2000        PMID: 10701835      PMCID: PMC312905     

Source DB:  PubMed          Journal:  Cell Stress Chaperones        ISSN: 1355-8145            Impact factor:   3.667


  18 in total

Review 1.  Molecular chaperones.

Authors:  R J Ellis; S M van der Vies
Journal:  Annu Rev Biochem       Date:  1991       Impact factor: 23.643

2.  Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections.

Authors:  S R Shi; M E Key; K L Kalra
Journal:  J Histochem Cytochem       Date:  1991-06       Impact factor: 2.479

Review 3.  Ovum factor and early pregnancy factor.

Authors:  H Morton; B E Rolfe; A C Cavanagh
Journal:  Curr Top Dev Biol       Date:  1987       Impact factor: 4.897

4.  Production and assay of dog, pig and goat heterologous anti-rabbit lymphocyte serum.

Authors:  H Morton; V Hegh; G J Clunie
Journal:  Clin Exp Immunol       Date:  1973-04       Impact factor: 4.330

5.  Effects of the modification of transfer buffer composition and the renaturation of proteins in gels on the recognition of proteins on Western blots by monoclonal antibodies.

Authors:  S D Dunn
Journal:  Anal Biochem       Date:  1986-08-15       Impact factor: 3.365

6.  Early pregnancy factor is immunosuppressive.

Authors:  F P Noonan; W J Halliday; H Morton; G J Clunie
Journal:  Nature       Date:  1979-04-12       Impact factor: 49.962

7.  Passive immunization of pregnant mice against early pregnancy factor causes loss of embryonic viability.

Authors:  S Athanasas-Platsis; K A Quinn; T Y Wong; B E Rolfe; A C Cavanagh; H Morton
Journal:  J Reprod Fertil       Date:  1989-11

8.  The human early pregnancy factor/chaperonin 10 gene family.

Authors:  K M Summers; R M Murphy; G C Webb; G B Peters; H Morton; A I Cassady; A C Cavanagh
Journal:  Biochem Mol Med       Date:  1996-06

9.  Active immunization with EPF suppresses the formation of immune ascites in BALB/c mice.

Authors:  K A Quinn
Journal:  Immunol Cell Biol       Date:  1991-02       Impact factor: 5.126

10.  Monoclonal antibodies to early pregnancy factor perturb tumour cell growth.

Authors:  K A Quinn; S Athanasas-Platsis; T Y Wong; B E Rolfe; A C Cavanagh; H Morton
Journal:  Clin Exp Immunol       Date:  1990-04       Impact factor: 4.330

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  3 in total

1.  Cross-reactivity between Chlamydia trachomatis heat shock protein 10 and early pregnancy factor.

Authors:  Fotini Betsou; Maria José Borrego; Nicolas Guillaume; Maria Anjos Catry; Sandra Romão; J A Machado-Caetano; Jean Marie Sueur; Jacques Mention; Nicole Faille; Jeanne Orfila
Journal:  Clin Diagn Lab Immunol       Date:  2003-05

2.  Systematic Review of Postfertilization Effects and Potential for Embryo Formation and Loss during the Use of Intrauterine Devices.

Authors:  Cara Buskmiller; Donna Harrison; Lester A Ruppersberger; Patrick P Yeung
Journal:  Linacre Q       Date:  2019-06-27

3.  Type I collagen synthesis by human osteoblasts in response to placental lactogen and chaperonin 10, a homolog of early-pregnancy factor.

Authors:  J P Mansell; S J Yarram; N L Brown; J R Sandy
Journal:  In Vitro Cell Dev Biol Anim       Date:  2002-10       Impact factor: 2.723

  3 in total

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