Literature DB >> 1069991

Brain tubulin polymerization in the absence of "microtubule-associated proteins".

R H Himes, P R Burton, R N Kersey, G B Pierson.   

Abstract

Pure tubulin dimer, purified by phosphecellulose chromatography to remove microtubule associated proteins, can be assembled into microtubules in the presence of dimethyl sulfoxide. The surfaces of such microtubules are devoid of filamentous material. The reaction is rapid and strongly dependent on protein concentration. No microtubule formation occurs from the purified tubulin when dimethyl sulfoxide is omitted.

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Year:  1976        PMID: 1069991      PMCID: PMC431471          DOI: 10.1073/pnas.73.12.4397

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN: 0027-8424            Impact factor:   11.205


  23 in total

1.  Tubulin requires an accessory protein for self assembly in microtubules.

Authors:  R A Keates; R H Hall
Journal:  Nature       Date:  1975-10-02       Impact factor: 49.962

2.  A protein factor essential for microtubule assembly.

Authors:  M D Weingarten; A H Lockwood; S Y Hwo; M W Kirschner
Journal:  Proc Natl Acad Sci U S A       Date:  1975-05       Impact factor: 11.205

3.  Association of high-molecular-weight proteins with microtubules and their role in microtubule assembly in vitro.

Authors:  D B Murphy; G G Borisy
Journal:  Proc Natl Acad Sci U S A       Date:  1975-07       Impact factor: 11.205

4.  Purification of tubulin and associated high molecular weight proteins from porcine brain and characterization of microtubule assembly in vitro.

Authors:  G G Borisy; J M Marcum; J B Olmsted; D B Murphy; K A Johnson
Journal:  Ann N Y Acad Sci       Date:  1975-06-30       Impact factor: 5.691

5.  The role of rings in the assembly of microtubules in vitro.

Authors:  M W Kirschner; M Suter; M Weingarten; D Littman
Journal:  Ann N Y Acad Sci       Date:  1975-06-30       Impact factor: 5.691

6.  Inhibition of tubulin assembly by RNA and other polyanions: evidence for a required protein.

Authors:  J B Bryan; B W Nagle; K H Doenges
Journal:  Proc Natl Acad Sci U S A       Date:  1975-09       Impact factor: 11.205

7.  Proteins associated with tubulin.

Authors:  I V Sandoval; P Cuatrecasas
Journal:  Biochem Biophys Res Commun       Date:  1976-01-12       Impact factor: 3.575

8.  Wetting agents for biological electron microscopy. I. General considerations and negative staining.

Authors:  D W Gregory; B J Pirie
Journal:  J Microsc       Date:  1973-12       Impact factor: 1.758

9.  Directionality of brain microtubule assembly in vitro.

Authors:  W L Dentler; S Granett; G B Witman; J L Rosenbaum
Journal:  Proc Natl Acad Sci U S A       Date:  1974-05       Impact factor: 11.205

10.  Ultrastructural localization of the high molecular weight proteins associated with in vitro-assembled brain microtubules.

Authors:  W L Dentler; S Granett; J L Rosenbaum
Journal:  J Cell Biol       Date:  1975-04       Impact factor: 10.539

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  17 in total

1.  Immunoflourescent staining of cytoplasmic and spindle microtubules in mouse fibroblasts with antibody to tau protein.

Authors:  J A Connolly; V I Kalnins; D W Cleveland; M W Kirschner
Journal:  Proc Natl Acad Sci U S A       Date:  1977-06       Impact factor: 11.205

2.  Differential turnover of tyrosinated and detyrosinated microtubules.

Authors:  D R Webster; G G Gundersen; J C Bulinski; G G Borisy
Journal:  Proc Natl Acad Sci U S A       Date:  1987-12       Impact factor: 11.205

3.  Association of mitochondria with microtubules in cultured cells.

Authors:  M H Heggeness; M Simon; S J Singer
Journal:  Proc Natl Acad Sci U S A       Date:  1978-08       Impact factor: 11.205

4.  Polarity of axoplasmic microtubules in the olfactory nerve of the frog.

Authors:  P R Burton; J L Paige
Journal:  Proc Natl Acad Sci U S A       Date:  1981-05       Impact factor: 11.205

5.  Species-dependent immunological differences between vertebrate brain tubulins.

Authors:  J L Morgan; C R Holladay; B S Spooner
Journal:  Proc Natl Acad Sci U S A       Date:  1978-03       Impact factor: 11.205

6.  An antimicrobial peptide regulates tumor-associated macrophage trafficking via the chemokine receptor CCR2, a model for tumorigenesis.

Authors:  Ge Jin; Hameem I Kawsar; Stanley A Hirsch; Chun Zeng; Xun Jia; Zhimin Feng; Santosh K Ghosh; Qing Yin Zheng; Aimin Zhou; Thomas M McIntyre; Aaron Weinberg
Journal:  PLoS One       Date:  2010-06-08       Impact factor: 3.240

7.  Axonal microtubules necessary for generation of sodium current in squid giant axons: I. Pharmacological study on sodium current and restoration of sodium current by microtubule proteins and 260K protein.

Authors:  G Matsumoto; M Ichikawa; A Tasaki; H Murofushi; H Sakai
Journal:  J Membr Biol       Date:  1984       Impact factor: 1.843

8.  Dimethyl sulfoxide-induced augmentation of adenosine-adenylate cyclase response of pig skin epidermis.

Authors:  M Watanabe; H Iizuka
Journal:  Arch Dermatol Res       Date:  1986       Impact factor: 3.017

9.  Influence of the centrosome on the structure of nucleated microtubules.

Authors:  L Evans; T Mitchison; M Kirschner
Journal:  J Cell Biol       Date:  1985-04       Impact factor: 10.539

10.  Self-organization of stabilized microtubules by both spindle and midzone mechanisms in Xenopus egg cytosol.

Authors:  Timothy J Mitchison; Phuong Nguyen; Margaret Coughlin; Aaron C Groen
Journal:  Mol Biol Cell       Date:  2013-03-20       Impact factor: 4.138

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