Prior electrical stimulation of the inferior colliculus sensitizes rats to the stress of the elevated plus-maze test.

Besides its primary function in the transmission of acoustic signals, the inferior colliculus (IC) is involved in conveying auditory information of aversive nature to higher cortical structures. In the field of anxiety research, one widely used animal model is the electrical stimulation of a given structure supposed to be involved in the neural circuitry underlying emotional behavior. Indeed, electrical stimulation of the inferior colliculus produces fear-like responses. Moreover, prior exposure to stressful events sensitizes rodents' responsivity to fearful stimuli when they are subsequently tested in the elevated plus-maze. Based on these evidence it seems to be important to know whether animals stimulated in the inferior colliculus would show a heightened behavioral responsivity to subsequent stressful events. To this end, we examined the temporal course of the effects of the electrical stimulation of this midbrain region (5, 10 and 15 min afterward) on the conventional and ethological measures of the behavior of rats exposed to the elevated plus-maze test. Prior electrical stimulation of the inferior colliculus produced an 'anxiogenic' profile in the elevated plus-maze, i.e. a significant reduction in the number of entries and time spent into the open arms. Still, previous electrical stimulation of the inferior colliculus caused a significant decrease in rearing, scanning, peeping out, head dipping and end-arm activity while increased immobility. All these changes occurred after 5 min of inferior colliculus electrical stimulation. Therefore, stimulation of the inferior colliculus causes a heightened responsivity to anxiogenic stimuli inherent to the elevated plus-maze test. These findings bring additional support to the proposed role of this midbrain structure in the elaboration of adaptive responses to stressful situations.