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Literature DB >> 10699044

Rapid subtyping of dengue virus serotypes 1 and 4 by restriction site-specific PCR.

M P Miagostovich¹, F B dos Santos, C M Gutiérrez, L W Riley, E Harris.

Abstract

We previously reported a simple subtyping method, restriction site-specific PCR (RSS-PCR), for dengue virus serotypes 2 and 3; here we describe its application for subtyping dengue virus serotypes 1 and 4. Three major RSS-PCR types were observed for dengue virus serotype 1 and two types were observed for dengue virus serotype 4, in agreement with previous strain classifications based on sequence analysis. Because of its simplicity, this method is amenable to rapid subtyping and application to epidemiological studies of dengue in countries where dengue is endemic.

Entities: Species

Mesh：

Substances：
DNA Primers

Year: 2000 PMID： 10699044 PMCID： PMC88609

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

18 in total

1. Molecular evolution and distribution of dengue viruses type 1 and 2 in nature.

Authors: R Rico-Hesse
Journal: Virology Date: 1990-02 Impact factor: 3.616

2. Rapid subtyping of dengue viruses by restriction site-specific (RSS)-PCR.

Authors: E Harris; E Sandoval; A M Xet-Mull; M Johnson; L W Riley
Journal: Virology Date: 1999-01-05 Impact factor: 3.616

Review 3. Flavivirus genome organization, expression, and replication.

Authors: T J Chambers; C S Hahn; R Galler; C M Rice
Journal: Annu Rev Microbiol Date: 1990 Impact factor: 15.500

4. Genetic relatedness among structural protein genes of dengue 1 virus strains.

Authors: M C Chu; E J O'Rourke; D W Trent
Journal: J Gen Virol Date: 1989-07 Impact factor: 3.891

5. Direct sequencing of genomic cDNA fragments amplified by the polymerase chain reaction for molecular epidemiology of dengue-2 viruses.

Authors: V Deubel; R M Nogueira; M T Drouet; H Zeller; J M Reynes; D Q Ha
Journal: Arch Virol Date: 1993 Impact factor: 2.574

1. Nested restriction site-specific PCR to detect and type hepatitis C virus (HCV): a rapid method to distinguish HCV subtype 1b from other genotypes.

Authors: L Krekulova; V Rehak; A E Wakil; E Harris; L W Riley
Journal: J Clin Microbiol Date: 2001-05 Impact factor: 5.948

1 in total

Rapid subtyping of dengue virus serotypes 1 and 4 by restriction site-specific PCR.

1. Molecular evolution and distribution of dengue viruses type 1 and 2 in nature.

2. Rapid subtyping of dengue viruses by restriction site-specific (RSS)-PCR.

Review 3. Flavivirus genome organization, expression, and replication.

4. Genetic relatedness among structural protein genes of dengue 1 virus strains.

5. Direct sequencing of genomic cDNA fragments amplified by the polymerase chain reaction for molecular epidemiology of dengue-2 viruses.

6. Phylogenetic relationships of dengue-2 viruses.

7. Molecular evolution and epidemiology of dengue-3 viruses.

8. Mosquito cell cultures and specific monoclonal antibodies in surveillance for dengue viruses.

Review 9. Mosquito cell cultures and the study of arthropod-borne togaviruses.

10. Genetic variation and microevolution of dengue 2 virus in Southeast Asia.

1. Nested restriction site-specific PCR to detect and type hepatitis C virus (HCV): a rapid method to distinguish HCV subtype 1b from other genotypes.