Literature DB >> 10698796

Development of a nonantibiotic dominant marker for positively selecting expression plasmids in multivalent Salmonella vaccines.

H V McNeill1, K A Sinha, C E Hormaeche, J J Lee, C M Khan.   

Abstract

We report the novel application of a herbicide-resistance-based dominant marker for the positive selection of expression plasmids in Salmonella serovar vaccines. The beta-lactamase gene of the plasmid pTETnir15, which expresses fragment C of tetanus toxin (TetC), has been replaced with the bar gene marker. The new plasmid pBAT1 can be positively selected in vitro within Salmonella serovars in the presence of the herbicide DL-phosphinothricin. The expression of TetC remains unaltered, and the Salmonella enterica serovar Typhimurium vaccine strain is stable and immunogenic in vivo.

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Year:  2000        PMID： 10698796      PMCID： PMC91967          DOI： 10.1128/AEM.66.3.1216-1219.2000

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  16 in total

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Journal:  Lancet       Date:  1988-08-27       Impact factor: 79.321

2.  An attenuated aroA Salmonella typhimurium vaccine elicits humoral and cellular immunity to cloned beta-galactosidase in mice.

Authors:  A Brown; C E Hormaeche; R Demarco de Hormaeche; M Winther; G Dougan; D J Maskell; B A Stocker
Journal:  J Infect Dis       Date:  1987-01       Impact factor: 5.226

3.  Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

Authors:  R K Saiki; D H Gelfand; S Stoffel; S J Scharf; R Higuchi; G T Horn; K B Mullis; H A Erlich
Journal:  Science       Date:  1988-01-29       Impact factor: 47.728

4.  A chromosomal integration system for stabilization of heterologous genes in Salmonella based vaccine strains.

Authors:  D Hone; S Attridge; L van den Bosch; J Hackett
Journal:  Microb Pathog       Date:  1988-12       Impact factor: 3.738

5.  Aromatic-dependent Salmonella typhimurium are non-virulent and effective as live vaccines.

Authors:  S K Hoiseth; B A Stocker
Journal:  Nature       Date:  1981-05-21       Impact factor: 49.962

6.  Construction, expression, and immunogenicity of the Schistosoma mansoni P28 glutathione S-transferase as a genetic fusion to tetanus toxin fragment C in a live Aro attenuated vaccine strain of Salmonella.

Authors:  C M Khan; B Villarreal-Ramos; R J Pierce; G Riveau; R Demarco de Hormaeche; H McNeill; T Ali; N Fairweather; S Chatfield; A Capron
Journal:  Proc Natl Acad Sci U S A       Date:  1994-11-08       Impact factor: 11.205

7.  Safety, infectivity, immunogenicity, and in vivo stability of two attenuated auxotrophic mutant strains of Salmonella typhi, 541Ty and 543Ty, as live oral vaccines in humans.

Authors:  M M Levine; D Herrington; J R Murphy; J G Morris; G Losonsky; B Tall; A A Lindberg; S Svenson; S Baqar; M F Edwards
Journal:  J Clin Invest       Date:  1987-03       Impact factor: 14.808

8.  Site-specific deletions of chromosomally located DNA segments with the multimer resolution system of broad-host-range plasmid RP4.

Authors:  C S Kristensen; L Eberl; J M Sanchez-Romero; M Givskov; S Molin; V De Lorenzo
Journal:  J Bacteriol       Date:  1995-01       Impact factor: 3.490

9.  Salmonella typhi vaccine strain CVD 908 expressing the circumsporozoite protein of Plasmodium falciparum: strain construction and safety and immunogenicity in humans.

Authors:  C Gonzalez; D Hone; F R Noriega; C O Tacket; J R Davis; G Losonsky; J P Nataro; S Hoffman; A Malik; E Nardin; M B Sztein; D G Heppner; T R Fouts; A Isibasi; M M Levine
Journal:  J Infect Dis       Date:  1994-04       Impact factor: 5.226

10.  Construction of a genetically defined Salmonella typhi Ty2 aroA, aroC mutant for the engineering of a candidate oral typhoid-tetanus vaccine.

Authors:  S N Chatfield; N Fairweather; I Charles; D Pickard; M Levine; D Hone; M Posada; R A Strugnell; G Dougan
Journal:  Vaccine       Date:  1992       Impact factor: 3.641
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  4 in total

1.  Stabilization of a plasmid coding for a heterologous antigen in Salmonella enterica serotype typhi vaccine strain CVD908-htrA by using site-specific recombination.

Authors:  Jonathan C Stephens; Michael J Darsley; Arthur K Turner
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2.  Antibiotic-free plasmid stabilization by operator-repressor titration for vaccine delivery by using live Salmonella enterica Serovar typhimurium.

Authors:  Helen S Garmory; Matthew W Leckenby; Kate F Griffin; Stephen J Elvin; Rosa R Taylor; M Gill Hartley; Julian A J Hanak; E Diane Williamson; Rocky M Cranenburgh
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3.  Use of mchI encoding immunity to the antimicrobial peptide microcin H47 as a plasmid selection marker in attenuated bacterial live vectors.

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Journal:  Infect Immun       Date:  2008-07-28       Impact factor: 3.441

4.  Development of non-antibiotic-resistant, chromosomally based, constitutive and inducible expression systems for aroA-attenuated Salmonella enterica Serovar Typhimurium.

Authors:  Jake N Matic; Tamsin D Terry; David Van Bockel; Tracy Maddocks; David Tinworth; Michael P Jennings; Steven P Djordjevic; Mark J Walker
Journal:  Infect Immun       Date:  2009-02-17       Impact factor: 3.441
  4 in total

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