| Literature DB >> 10698313 |
P Schuck1, C G Radu, E S Ward.
Abstract
The interaction of mouse IgG1 or IgG1-derived Fc fragment with recombinant, insect cell expressed mouse FcRn has been analyzed using sedimentation equilibrium. This results in a model for the interaction in which the two binding sites for FcRn on Fc or IgG1 have significantly different affinities with macroscopic binding constants of < 130 nM and 6 microM. This data indicates the formation of an asymmetric FcRn:Fc (or IgG1):FcRn complex which is consistent with earlier suggestions that for this form of recombinant FcRn, binding to IgG1 or Fc does not result in a symmetric 2:1 complex in which both binding sites are equivalent.Entities:
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Year: 1999 PMID： 10698313 DOI： 10.1016/s0161-5890(99)00093-0
Source DB: PubMed Journal: Mol Immunol ISSN： 0161-5890 Impact factor: 4.407