| Literature DB >> 10695806 |
B D Pandey1, N Karabatsos, B Cropp, M Tagaki, Y Tsuda, A Ichinose, A Igarashi.
Abstract
A virus isolate, ThCAr105/92, from a pool of mosquitos, Culex tritaeniorhynchus, collected in Chiang Mai, Thailand in 1992, appeared to be a member of the genus Flavivirus of the family Flaviviridae, based on the reverse transcription polymerase chain reaction (RT-PCR) using flavivirus cross-reacting primer pairs, electron microscopic examination, and serological tests. However, RT-PCR using Japanese encephalitis (JE) virus-specific primers showed that the isolate was different from JE virus. Sucrose density gradient sedimentation of the virus replicated in C6/36 cells indicated that the virus is relatively unstable in the infected culture fluids at 37 degrees C. Antibody prepared against this virus and a virus seed for the isolate were tested by cross neutralization against a panel of flaviviruses and the results showed that the new isolate was a distinct subtype of Tembusu virus.Entities:
Mesh：
Substances：
Year: 1999 PMID： 10695806
Source DB: PubMed Journal: Southeast Asian J Trop Med Public Health ISSN： 0125-1562 Impact factor: 0.267