P Selvaraj1, P R Narayanan, A M Reetha. 1. Tuberculosis Research Centre (ICMR), Chennai, India. icmrtrc@ren.nic.in/trcicmr@giasmd01.vsnl.net.in
Abstract
SETTING: Mannose binding protein gene polymorphism in pulmonary tuberculosis in India. OBJECTIVE: To find out whether non-HLA genes such as mannose binding protein (MBP) genes are associated in the susceptibility to pulmonary tuberculosis. DESIGN: Genotyping of MBP 52, 54 and 57 wild and mutant alleles was carried out in HLA-DR typed pulmonary tuberculosis patients (n = 202) and control subjects (n = 109). Since HLA-DR2 is associated with pulmonary-TB, the interaction of MBP genes on -DR2 and non-DR2 genes on the susceptibility was also studied. RESULTS: A significantly increased genotype frequency of MBP functional mutant homozygotes (including 52, 54 and 57) was seen in pulmonary tuberculosis (PTB) patients (10.9%) than in control subjects (1.8%; P = 0.008; odds ratio: 6.5). Analysis of interaction of MBP genes and HLA-DR2 on the susceptibility to PTB revealed that these genes are associated with PTB independent of each other. CONCLUSION: The present study shows that functional mutants of MBP are associated with PTB. Apart from HLA-DR2 association, association of non-HLA genes in the susceptibility to PTB is evident. This suggests that multigenetic factors (candidate genes) may be involved in the susceptibility/resistance to PTB.
SETTING:Mannose binding protein gene polymorphism in pulmonary tuberculosis in India. OBJECTIVE: To find out whether non-HLA genes such as mannose binding protein (MBP) genes are associated in the susceptibility to pulmonary tuberculosis. DESIGN: Genotyping of MBP 52, 54 and 57 wild and mutant alleles was carried out in HLA-DR typed pulmonary tuberculosispatients (n = 202) and control subjects (n = 109). Since HLA-DR2 is associated with pulmonary-TB, the interaction of MBP genes on -DR2 and non-DR2 genes on the susceptibility was also studied. RESULTS: A significantly increased genotype frequency of MBP functional mutant homozygotes (including 52, 54 and 57) was seen in pulmonary tuberculosis (PTB) patients (10.9%) than in control subjects (1.8%; P = 0.008; odds ratio: 6.5). Analysis of interaction of MBP genes and HLA-DR2 on the susceptibility to PTB revealed that these genes are associated with PTB independent of each other. CONCLUSION: The present study shows that functional mutants of MBP are associated with PTB. Apart from HLA-DR2 association, association of non-HLA genes in the susceptibility to PTB is evident. This suggests that multigenetic factors (candidate genes) may be involved in the susceptibility/resistance to PTB.
Authors: Susan E Dorman; Christine L Hatem; Sandeep Tyagi; Katherine Aird; Javier Lopez-Molina; M Louise M Pitt; Bernard C Zook; Arthur M Dannenberg; William R Bishai; Yukari C Manabe Journal: Infect Immun Date: 2004-03 Impact factor: 3.441