Literature DB >> 10692589

Isolation of recombinant BMP receptor IA ectodomain and its 2:1 complex with BMP-2.

T Kirsch1, J Nickel, W Sebald.   

Abstract

Bone morphogenetic protein-2 (BMP-2) is a member of the transforming growth factor beta superfamily which induces bone formation and regeneration, and important steps during early embryonic development. BMP-2 signals via oligomerization of type I and type II serine/threonine kinase receptors. We report here expression of the extracellular domain of the human type IA receptor for BMP-2 (BMPR-IA) in Escherichia coli. This soluble form of BMPR-IA (sBMPR-IA) was purified employing a BMP-2 affinity column. Gel filtration experiments and analysis of gel filtration fractions by polyacrylamide electrophoresis and densitometry reveal that BMP-2 forms a defined 1:2 complex with sBMPR-IA that can be purified and hopefully used for crystallization studies.

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Year:  2000        PMID： 10692589     DOI： 10.1016/s0014-5793(00)01214-x

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  25 in total

1.  Crystallization of BMP receptor type IA bound to the antibody Fab fragment AbD1556.

Authors:  Stefan Harth; Alexander Kotzsch; Walter Sebald; Thomas Dieter Mueller
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2010-07-29

2.  Bone morphogenetic protein-2 and -6 heterodimer illustrates the nature of ligand-receptor assembly.

Authors:  Michael J Isaacs; Yasuhiko Kawakami; George P Allendorph; Byung-Hak Yoon; Juan Carlos Izpisua Belmonte; Senyon Choe
Journal:  Mol Endocrinol       Date:  2010-05-19

3.  Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry.

Authors:  Claudia Siverino; Barbara Tabisz; Tessa Lühmann; Lorenz Meinel; Thomas Müller; Heike Walles; Joachim Nickel
Journal:  J Vis Exp       Date:  2018-03-29       Impact factor: 1.355

4.  Effects of osteogenic growth factors on bone marrow stromal cell differentiation in a mineral-based delivery system.

Authors:  Linh N Luong; Janani Ramaswamy; David H Kohn
Journal:  Biomaterials       Date:  2011-10-19       Impact factor: 12.479

Review 5.  BMP signaling in vascular development and disease.

Authors:  Jonathan W Lowery; Mark P de Caestecker
Journal:  Cytokine Growth Factor Rev       Date:  2010-07-31       Impact factor: 7.638

6.  c-Abl-dependent molecular circuitry involving Smad5 and phosphatidylinositol 3-kinase regulates bone morphogenetic protein-2-induced osteogenesis.

Authors:  Nandini Ghosh-Choudhury; Chandi C Mandal; Falguni Das; Suthakar Ganapathy; Seema Ahuja; Goutam Ghosh Choudhury
Journal:  J Biol Chem       Date:  2013-07-02       Impact factor: 5.157

7.  Receptor oligomerization and beyond: a case study in bone morphogenetic proteins.

Authors:  Kai Heinecke; Axel Seher; Werner Schmitz; Thomas D Mueller; Walter Sebald; Joachim Nickel
Journal:  BMC Biol       Date:  2009-09-07       Impact factor: 7.431

8.  A selection fit mechanism in BMP receptor IA as a possible source for BMP ligand-receptor promiscuity.

Authors:  Stefan Harth; Alexander Kotzsch; Junli Hu; Walter Sebald; Thomas D Mueller
Journal:  PLoS One       Date:  2010-09-28       Impact factor: 3.240

9.  Functional analysis of saxophone, the Drosophila gene encoding the BMP type I receptor ortholog of human ALK1/ACVRL1 and ACVR1/ALK2.

Authors:  Vern Twombly; Erdem Bangi; Viet Le; Bettina Malnic; Matthew A Singer; Kristi A Wharton
Journal:  Genetics       Date:  2009-07-20       Impact factor: 4.562

10.  Purification, crystallization and preliminary data analysis of ligand-receptor complexes of growth and differentiation factor 5 (GDF5) and BMP receptor IB (BRIB).

Authors:  Alexander Kotzsch; Joachim Nickel; Walter Sebald; Thomas D Mueller
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2009-07-25
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