Literature DB >> 10691730

Isolation of the pharmacologically active saponin ginsenoside Rb1 from ginseng by immunoaffinity column chromatography.

N Fukuda1, H Tanaka, Y Shoyama.   

Abstract

Immunoaffinity column chromatography using an anti-ginsenoside Rb1 monoclonal antibody has made possible a single-step separation of ginsenoside Rb1 from a crude extract of ginseng roots (Panax ginseng). The combination of immunoaffinity column chromatography and an enzyme-linked immunosorbent assay (ELISA) was also investigated.

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Year:  2000        PMID： 10691730     DOI： 10.1021/np990356s

Source DB:  PubMed          Journal:  J Nat Prod        ISSN： 0163-3864            Impact factor:   4.050


  5 in total

1.  Preparation of DESIGNER extracts of red clover (Trifolium pratense L.) by centrifugal partition chromatography.

Authors:  Gonzalo R Malca Garcia; J Brent Friesen; Yang Liu; Dejan Nikolić; David C Lankin; James B McAlpine; Shao-Nong Chen; Guido F Pauli
Journal:  J Chromatogr A       Date:  2019-05-29       Impact factor: 4.759

2.  Preparation of anti-2,4-dichlorophenol and 2,4-dichlorophenoxyacetic acid monoclonal antibodies.

Authors:  Hiroyuki Tanaka; Shayu Yan; Norio Miura; Yukihiro Shoyama
Journal:  Cytotechnology       Date:  2003-07       Impact factor: 2.058

3.  Application of Monoclonal Antibodies against Bioactive Natural Products: Eastern Blotting and Preparation of Knockout Extract.

Authors:  Hiroyuki Tanaka; Osamu Morinaga; Takuhiro Uto; Shunsuke Fuji; Frederick Asare Aboagye; Nguyen Huu Tung; Xiao Wei Li; Waraporn Putalun; Yukihiro Shoyama
Journal:  Int J Anal Chem       Date:  2012-02-14       Impact factor: 1.885

4.  K-targeted metabolomic analysis extends chemical subtraction to DESIGNER extracts: selective depletion of extracts of hops (Humulus lupulus).

Authors:  René F Ramos Alvarenga; J Brent Friesen; Dejan Nikolić; Charlotte Simmler; José G Napolitano; Richard van Breemen; David C Lankin; James B McAlpine; Guido F Pauli; Shao-Nong Chen
Journal:  J Nat Prod       Date:  2014-12-01       Impact factor: 4.050

Review 5.  Immunological Separation of Bioactive Natural Compounds from Crude Drug Extract and Its Application for Cell-Based Studies.

Authors:  Takuhiro Uto; Tomoe Ohta; Shunsuke Fujii; Yukihiro Shoyama
Journal:  Antibodies (Basel)       Date:  2021-12-06
  5 in total

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