F Medina1, C Segundo, J A Brieva. 1. Servicio de Inmunología, Hospital Universitario Puerta del Mar, Cádiz, Spain.
Abstract
BACKGROUND: The advancement of knowledge about the biology of human normal plasma cells (PC) is hampered by their low frequency and difficult isolation. The aim of this study is to design a way of purifying these cells. METHODS: To this end, advantage was taken of the fact that human tonsil PC expressed surface CD31 at higher levels than the rest of tonsil B cells. RESULTS: The immunomagnetic selection of CD31(+) cells from tonsil B cells increased by a factor of 12 the proportion of PC, determined as CD38(high) cells. This method recovered half of the initial number of PC, and did not alter the PC functions, because IgG secretion was similar in control B cell cultures as well as in cultures of B cells obtained at successive steps of the selection procedure. In addition, CD38(high) cells pre-enriched by this technique were readily isolated by FACS sorting and clearly identified as PC. CONCLUSIONS: Therefore, immunomagnetic pre-enrichment of CD31(+) cells is an efficient method that allows the complete purification of human functional PC. Copyright 2000 Wiley-Liss, Inc.
BACKGROUND: The advancement of knowledge about the biology of human normal plasma cells (PC) is hampered by their low frequency and difficult isolation. The aim of this study is to design a way of purifying these cells. METHODS: To this end, advantage was taken of the fact that human tonsil PC expressed surface CD31 at higher levels than the rest of tonsil B cells. RESULTS: The immunomagnetic selection of CD31(+) cells from tonsil B cells increased by a factor of 12 the proportion of PC, determined as CD38(high) cells. This method recovered half of the initial number of PC, and did not alter the PC functions, because IgG secretion was similar in control B cell cultures as well as in cultures of B cells obtained at successive steps of the selection procedure. In addition, CD38(high) cells pre-enriched by this technique were readily isolated by FACS sorting and clearly identified as PC. CONCLUSIONS: Therefore, immunomagnetic pre-enrichment of CD31(+) cells is an efficient method that allows the complete purification of human functional PC. Copyright 2000 Wiley-Liss, Inc.
Authors: Maria D Jimenez-Nuñez; David Moreno-Sanchez; Laura Hernandez-Ruiz; Alicia Benítez-Rondán; Ana Ramos-Amaya; Beatriz Rodríguez-Bayona; Francisco Medina; José Antonio Brieva; Felix A Ruiz Journal: Haematologica Date: 2012-02-07 Impact factor: 9.941
Authors: John De Vos; Dirk Hose; Thierry Rème; Karin Tarte; Jérôme Moreaux; Karéne Mahtouk; Michel Jourdan; Hartmut Goldschmidt; Jean-François Rossi; Friedrich W Cremer; Bernard Klein Journal: Immunol Rev Date: 2006-04 Impact factor: 12.988