Lesion-induced increase of BDNF is greater in the striatum of young versus old rat brain.

Young (4-5 month old) and old (32-34 month old) Brown Norway/F344 hybrid rats were given unilateral 6-OHDA lesions of the nigrostriatal pathway. Four weeks later tissue from the lesioned or intact striatum or ventral midbrain was dissected and analyzed for brain-derived neurotrophic factor (BDNF) protein levels using an enzyme-linked immunosorbent assay. BDNF protein content was greater in the lesioned striatum than in the intact striatum for all young rats, and the increased BDNF content in the lesioned striatum of young rats was directly correlated with severity of lesion as determined by rotational scores. BDNF content in the lesioned striatum increased in less than half of the old rats and was not significantly different than BDNF content in the intact striatum. BDNF content in the lesioned substantia nigra/ventral tegmental area (SN/VTA) was greater than BDNF content in the intact SN/VTA for both young and old rats. These data suggest that an age-related difference in activity of at least one neurotrophic factor, BDNF, occur within the denervated striatum following a neurotoxic lesion of the nigrostriatal pathway. Copyright 2000 Academic Press.