C Fu1, Q Zhang, F Lu, Z Zhang, Y Hu, L Lin. 1. Department of Gynecology and Obstetry, Second Affiliated Hospital, Hunan Medical University, Changsha.
Abstract
OBJECTIVE: To evaluate the relationship between the regulatory factors in G1 phase of cell cycle and the cacrinogenesis of the trophoblastic cells. METHODS: The expressions of p16, cyclin-dependent kinase 4 (CDK4), proliferation cell nuclear antigen (PCNA) proteins in 18 cases of trophoblastic tumors, 30 cases of hydatidiform mole and 30 cases of normal villi were studied by immunohistochemical methods. RESULTS: The expressions of p16 protein between malignant trophoblastic tumors and normal villi were significantly different (P < 0.05). PCNA positive rate in p16 positive samples were markedly lower than that in negative samples (P < 0.05). The expressions of CDK4 among normal villi, hydatiform mole and trophoblastic tumors were not significantly different. Patients with positive p16 expression had a higher 3 year survival rate. CONCLUSION: p16 protein may inhibit trophoblastic cells proliferation, and p16 gene mutation may be an important factor in carcinogenesis of trophoblastic cells and proliferation out of control. P16 protein expression examination is helpful in prediction of patients prognosis.
OBJECTIVE: To evaluate the relationship between the regulatory factors in G1 phase of cell cycle and the cacrinogenesis of the trophoblastic cells. METHODS: The expressions of p16, cyclin-dependent kinase 4 (CDK4), proliferation cell nuclear antigen (PCNA) proteins in 18 cases of trophoblastic tumors, 30 cases of hydatidiform mole and 30 cases of normal villi were studied by immunohistochemical methods. RESULTS: The expressions of p16 protein between malignant trophoblastic tumors and normal villi were significantly different (P < 0.05). PCNA positive rate in p16 positive samples were markedly lower than that in negative samples (P < 0.05). The expressions of CDK4 among normal villi, hydatiform mole and trophoblastic tumors were not significantly different. Patients with positive p16 expression had a higher 3 year survival rate. CONCLUSION:p16 protein may inhibit trophoblastic cells proliferation, and p16 gene mutation may be an important factor in carcinogenesis of trophoblastic cells and proliferation out of control. P16 protein expression examination is helpful in prediction of patients prognosis.
Authors: Wei-Cheng Xue; Kelvin Y K Chan; Hui-Chen Feng; Pui-Man Chiu; Hextan Y S Ngan; Sai-Wah Tsao; Annie N Y Cheung Journal: J Mol Diagn Date: 2004-11 Impact factor: 5.568
Authors: Michelle K Y Siu; Matthew C W Yeung; HuiJuan Zhang; Daniel S H Kong; Joanna W K Ho; Hextan Y S Ngan; Dominic C W Chan; Annie N Y Cheung Journal: Am J Pathol Date: 2010-04-22 Impact factor: 4.307