Literature DB >> 10681029

Performance of the ELISA test for swine cysticercosis using antigens of Taenia solium and Taenia crassiceps cysticerci.

P S Pinto1, A J Vaz, P M Germano, P M Nakamura.   

Abstract

Studies were conducted to evaluate antigens of Taenia solium (Tso) and Taenia crassiceps (Tcra) cysticerci in the ELISA test for the diagnosis of swine cysticercosis. The samples analyzed were cysticercosis positive and negative control sera and heterologous sera. Four antigens were assayed: vesicular fluid (VF) and crude (T) Tcra and scolex (S) and crude (T) Tso. All antigens showed good performance, but VF-Tcra was the best followed by T-Tcra. Sensitivity rates of ELISA were respectively, in 2nd and 3rd standard deviation cut-offs, 96.0 and 80.0% for the VF antigen and specificity of 97.5 and 100.0%. Cross-reactivity was verified only for hidatidosis and ascaridiosis. Due to the high performance observed, the ELISA test using Tcra antigens should be recommended for the diagnosis of swine cysticercosis.

Entities:  

Mesh:

Substances:

Year:  2000        PMID: 10681029     DOI: 10.1016/s0304-4017(99)00201-0

Source DB:  PubMed          Journal:  Vet Parasitol        ISSN: 0304-4017            Impact factor:   2.738


  3 in total

1.  Cysticercosis immunodiagnosis using 18- and 14-kilodalton proteins from Taenia crassiceps cysticercus antigens obtained by immunoaffinity chromatography.

Authors:  Noeli Maria Espíndola; Alberto Hiroshi Iha; Irene Fernandes; Osvaldo Massaiti Takayanagui; Luís Dos Ramos Machado; José Antônio Livramento; Antônio Augusto Mendes Maia; José Mauro Peralta; Adelaide José Vaz
Journal:  J Clin Microbiol       Date:  2005-07       Impact factor: 5.948

2.  Use of Taenia crassiceps cysticercus antigen preparations for detection of antibodies in cerebrospinal fluid samples from patients with neurocysticercosis (Taenia solium).

Authors:  Alessandra Xavier Pardini; Regina Helena Peralta; Adelaide José Vaz; Luis dos Ramos Machado; José Mauro Peralta
Journal:  Clin Diagn Lab Immunol       Date:  2002-01

3.  Relationship between serum antibodies and Taenia solium larvae burden in pigs raised in field conditions.

Authors:  Cesar M Gavidia; Manuela R Verastegui; Hector H Garcia; Teresa Lopez-Urbina; Victor C W Tsang; William Pan; Robert H Gilman; Armando E Gonzalez
Journal:  PLoS Negl Trop Dis       Date:  2013-05-02
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.