Literature DB >> 10681013

Determination of reducing end sugar residues in oligo- and polysaccharides by gas-liquid chromatography.

C M Courtin1, H Van den Broeck, J A Delcour.   

Abstract

Reducing end sugar residues in maltodextrins and arabinoxylans are determined as alditol acetates by gas-liquid chromatography following reduction, acid hydrolysis and acetylation of the samples. After this conversion to alditol acetates, the reducing end sugars are thus separated from their acetylated aldose counterparts. The method allows to identify individual reducing end sugars quantitatively and is a good alternative for colorimetric reducing sugar assays and 1H-NMR analysis. To demonstrate the advantages of the method, an application in a study of enzymic solubilisation and degradation of water unextractable arabinoxylan from a flour squeegee fraction is described.

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Year:  2000        PMID： 10681013     DOI： 10.1016/s0021-9673(99)01064-x

Source DB:  PubMed          Journal:  J Chromatogr A        ISSN： 0021-9673            Impact factor:   4.759


  7 in total

1.  Analysis of the hydrolysis of inulin using real time 1H NMR spectroscopy.

Authors:  Thomas Barclay; Milena Ginic-Markovic; Martin R Johnston; Peter D Cooper; Nikolai Petrovsky
Journal:  Carbohydr Res       Date:  2012-03-10       Impact factor: 2.104

2.  A randomised, double-blind, placebo controlled cross-over study to determine the gastrointestinal effects of consumption of arabinoxylan-oligosaccharides enriched bread in healthy volunteers.

Authors:  Gemma E Walton; Congyi Lu; Isabel Trogh; Filip Arnaut; Glenn R Gibson
Journal:  Nutr J       Date:  2012-06-01       Impact factor: 3.271

3.  Effect of suppression of arabinoxylan synthetic genes in wheat endosperm on chain length of arabinoxylan and extract viscosity.

Authors:  Jackie Freeman; Alison Lovegrove; Mark David Wilkinson; Luc Saulnier; Peter Robert Shewry; Rowan Andrew Craig Mitchell
Journal:  Plant Biotechnol J       Date:  2015-03-27       Impact factor: 9.803

4.  Wheat Bran Does Not Affect Postprandial Plasma Short-Chain Fatty Acids from 13C-inulin Fermentation in Healthy Subjects.

Authors:  Lise Deroover; Joran Verspreet; Anja Luypaerts; Greet Vandermeulen; Christophe M Courtin; Kristin Verbeke
Journal:  Nutrients       Date:  2017-01-20       Impact factor: 5.717

5.  Enzyme-Catalyzed Production of Potato Galactan-Oligosaccharides and Its Optimization by Response Surface Methodology.

Authors:  Mirian Angelene González-Ayón; Ángel Licea-Claveríe; José Benigno Valdez-Torres; Lorenzo A Picos-Corrales; Rosabel Vélez-de la Rocha; Juan Carlos Contreras-Esquivel; John M Labavitch; Josefa Adriana Sañudo-Barajas
Journal:  Materials (Basel)       Date:  2019-05-07       Impact factor: 3.623

6.  Novel xylan-degrading enzymes from polysaccharide utilizing loci of Prevotella copri DSM18205.

Authors:  Javier A Linares-Pastén; Johan Sebastian Hero; José Horacio Pisa; Cristina Teixeira; Margareta Nyman; Patrick Adlercreutz; M Alejandra Martinez; Eva Nordberg Karlsson
Journal:  Glycobiology       Date:  2021-11-18       Impact factor: 4.313

7.  Purification, Identification, and Characterization of an Endo-1,4-β-Xylanase from Wheat Malt.

Authors:  Zhaojun Peng; Yuhong Jin
Journal:  Molecules       Date:  2020-03-29       Impact factor: 4.411
  7 in total

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