| Literature DB >> 10680966 |
D K Oladepo1, P E Klapper, D Percival, P J Vallely.
Abstract
Evaluation was made of three enzyme-linked immunosorbent assay (ELISA) formats; varicella-zoster virus (VZV) indirect ELISA; VZV IgM capture using biotin and VZV IgM capture using peroxidase, for the detection of VZV-specific IgM antibodies in human sera. It was observed that there was no significant difference in sensitivity of detection using the three formats but there were important practical differences in the number of steps and hence time for assay completion between the three assay formats. All assays showed some cross-reactivity with sera containing anti-HSV1 antibodies.Entities:
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Year: 2000 PMID: 10680966 DOI: 10.1016/s0166-0934(99)00139-1
Source DB: PubMed Journal: J Virol Methods ISSN: 0166-0934 Impact factor: 2.014