Literature DB >> 10672968

A labelled avidin-biotin ELISA to detect antibodies to caprine arthritis-encephalitis virus in goats' sera.

R S Castro1, R C Leite, M Resende, A M Gouveia.   

Abstract

A labelled avidin-biotin ELISA (lab-ELISA) was developed and compared with indirect ELISA (i-ELISA) and agar-gel immunodiffusion assay (AGID) for its efficacy in detecting antibodies against caprine arthritis-encephalitis virus (CAEV) in goat sera. The enzyme immunoassays were standardized using 113 sera from CAEV-negative goat flocks. The tests were compared using the results from 339 serum samples. The lab-ELISA showed the greatest number of positive results (94/339) as compared with AGID (51) and i-ELISA (64). The comparison of the other two tests with the lab-ELISA showed an agreement of 87.3% with AGID and 90.6% with i-ELISA. The lab-ELISA may be useful for screening large populations for CAEV antibodies, in epidemiological surveys and in the control of caprine arthritis-encephalitis.

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Year:  1999        PMID： 10672968     DOI： 10.1023/a:1006370607924

Source DB:  PubMed          Journal:  Vet Res Commun        ISSN： 0165-7380            Impact factor:   2.459


  26 in total

1.  Caprine arthritis-encephalitis virus: detection of proviral DNA in lactoserum cells.

Authors:  P Russo; C Vitu; A Bourgogne; M Vignoni; G Abadie; V David; M Pépin
Journal:  Vet Rec       Date:  1997-05-03       Impact factor: 2.695

2.  Antigenic cross-reactivity between caprine arthritis-encephalitis, visna and progressive pneumonia viruses involves all virion-associated proteins and glycoproteins.

Authors:  R P Gogolewski; D S Adams; T C McGuire; K L Banks; W P Cheevers
Journal:  J Gen Virol       Date:  1985-06       Impact factor: 3.891

3.  Bacterial expression of the caprine arthritis-encephalitis virus gag and env proteins and their use in enzyme-linked immunosorbent assay.

Authors:  A Clavijo; J Thorsen
Journal:  Am J Vet Res       Date:  1995-07       Impact factor: 1.156

4.  Global survey of serological evidence of caprine arthritis-encephalitis virus infection.

Authors:  D S Adams; R E Oliver; E Ameghino; J C DeMartini; D W Verwoerd; D J Houwers; S Waghela; J R Gorham; B Hyllseth; M Dawson
Journal:  Vet Rec       Date:  1984-11-10       Impact factor: 2.695

5.  Delayed seroconversion following naturally acquired caprine arthritis-encephalitis virus infection in goats.

Authors:  E Rimstad; N E East; M Torten; J Higgins; E DeRock; N C Pedersen
Journal:  Am J Vet Res       Date:  1993-11       Impact factor: 1.156

6.  Double-nested polymerase chain reaction for detection of caprine arthritis-encephalitis virus proviral DNA in blood, milk, and tissues of infected goats.

Authors:  J Barlough; N East; J D Rowe; K Van Hoosear; E DeRock; L Bigornia; E Rimstad
Journal:  J Virol Methods       Date:  1994-12       Impact factor: 2.014

7.  An indirect enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to maedi-visna virus.

Authors:  D J Houwers; A L Gielkens; J Schaake
Journal:  Vet Microbiol       Date:  1982-07       Impact factor: 3.293

8.  Transmission and control of caprine arthritis-encephalitis virus.

Authors:  D S Adams; P Klevjer-Anderson; J L Carlson; T C McGuire; J R Gorham
Journal:  Am J Vet Res       Date:  1983-09       Impact factor: 1.156

9.  Detection of antibodies to caprine arthritis-encephalitis virus using recombinant gag proteins.

Authors:  E Rimstad; N East; E DeRock; J Higgins; N C Pedersen
Journal:  Arch Virol       Date:  1994       Impact factor: 2.574

10.  Preferential immune response to virion surface glycoproteins by caprine arthritis-encephalitis virus-infected goats.

Authors:  G C Johnson; A F Barbet; P Klevjer-Anderson; T C McGuire
Journal:  Infect Immun       Date:  1983-08       Impact factor: 3.441
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  1 in total

1.  An outbreak of visna-maedi in a flock of sheep in Southern Brazil.

Authors:  Bianca S Cecco; Luan C Henker; Marina P Lorenzett; Franciéli A Molossi; Claiton I Schwertz; Letícia F Baumbach; Matheus N Weber; Cláudio W Canal; David Driemeier; Saulo P Pavarini; Ingeborg M Langohr; Mariano Carossino; Udeni B R Balasuriya; Luciana Sonne
Journal:  Braz J Microbiol       Date:  2022-04-27       Impact factor: 2.214
  1 in total

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