| Literature DB >> 10669804 |
H J Yoon1, W Hashimoto, Y Katsuya, Y Mezaki, K Murata, B Mikami.
Abstract
Alginate lyase A1-II of Sphingomonas species A1 was purified and crystallized using the hanging drop vapor-diffusion method in 0.1 M Tris-HCl buffer containing 43% saturated ammonium sulfate, 8% polyethylene glycol 4000 and 0.2 M Li(2)SO(4) at pH 8.5 and 20 degrees C. The crystals are tetragonal and belong to the space group P4(3)2(1)2 or P4(1)2(1)2 with unit cell dimensions of a=b=144.07 and c=296.38 A. The diffraction data up to 2.8 A were collected by a synchrotron radiation source at SPring-8 in Japan.Mesh：
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Year: 2000 PMID： 10669804 DOI： 10.1016/s0167-4838(99)00244-7
Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002