| Literature DB >> 10669310 |
P C Schmid1, K D Schwartz, C N Smith, R J Krebsbach, E V Berdyshev, H H Schmid.
Abstract
Mammalian cells produce both N-arachidonoylethanolamine (20:4n-6 NAE, anandamide) and 2-arachidonoylglycerol (2-AG), lipid signaling molecules that activate cannabinoid receptors. Because both agonists occur in the presence of receptor-inactive congeners, we have developed a sensitive method for the simultaneous assay of N-acylethanolamines (NAEs) and 2-monoacylglycerols (2-MAG). These lipid classes are isolated from total lipids by solid phase extraction and converted to tert-butyldimethylsilyl (tBDMS) derivatives in the presence of deuterated analogs. The tBDMS derivatives are analyzed by gas chromatography/mass spectrometry using selected ion monitoring programs specific for NAE and 2-MAG. Individual NAEs and 2-MAGs can be quantified in the nanogram and subnanogram range. The NAE and 2-MAG compositions of rat organs and cultured JB6 cells are reported.Entities:
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Year: 2000 PMID： 10669310 DOI： 10.1016/s0009-3084(99)00124-3
Source DB: PubMed Journal: Chem Phys Lipids ISSN： 0009-3084 Impact factor: 3.329