| Literature DB >> 10669066 |
P Shapshak1, R K Fujimura, J B Page, D Segal, J E Rivers, J Yang, S M Shah, G Graham, L Metsch, N Weatherby, D D Chitwood, C B McCoy.
Abstract
We quantified HIV-1 RNA load in rinses from needles/syringes (N/S) obtained at shooting galleries in Miami and also analyzed the rinses for antibodies for viral proteins. In rinses from 36 N/S that contained visible blood, 14 (39%) had detectable amounts of HIV-1 RNA. Numbers of copies of HIV-1 RNA ranged from the detection limit (400 copies/ml) to 268,000 copies/ml. We also detected antibodies to HIV-1 polypeptides in 34/36 (94%) of rinses from visibly contaminated N/S using Western blots specific for the HIV-1 proteins. No antibodies were detected in laboratory rinses from six visibly clean needles. The presence of HIV-1 RNA in N/S is an important indication of the risk created by N/S sharing as well as by shared paraphernalia and wash waters by injecting drug users.Entities:
Mesh：
Substances：
Year: 2000 PMID： 10669066 DOI： 10.1016/s0376-8716(99)00085-x
Source DB: PubMed Journal: Drug Alcohol Depend ISSN： 0376-8716 Impact factor: 4.492