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Literature DB >> 1066686

Detection of mRNA sequences in nuclear 30S ribonucleoprotein subcomplexes.

Abstract

RNA from nuclear 30S ribonucleoprotein (RNP) complexes of mouse ascites cells has been shows to contain sequences homologous to poly(A) + mRNA by its ability to hybridize with complementary DNA prepared from poly(A) + mRNA template. Analysis of the hybridization kinetics of poly(A) + mRNA with its own complementary DNA revealed several abundancy classes. The total complexity of poly(A) + mRNA from ascites cells was estimated to be approximately 30,000 sequences of average molecular weight (6 X 10(5)). When the hybridization reaction of 30S RNP-RNA with mRNA-specific cDNA was compared to the homologous reaction the majority, and most probably all, of the poly(A) + mRNA sequences were found to be present in the RNA. The kinetics of hybridization suggest that 10-15% of the RNA in this RNP complex is homologous to poly(A) + mRNA. The 30S RNP subcomplexes therefore contain nuclear poly(A) + mRNA sequences as well as the bulk of heterogeneous RNA.

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Year: 1976 PMID： 1066686 PMCID： PMC430721 DOI： 10.1073/pnas.73.8.2725

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

35 in total

1. Proteins associated with globin messenger RNA in avian erythroblasts: Isolation and comparison with the proteins bound to nuclear messenger-likie RNA.

Authors: Carlos Morel; Boniface Kayibanda; Klaus Scherrer
Journal: FEBS Lett Date: 1971-10-15 Impact factor: 4.124

2. Polyadenylic acid sequences in ascites cells nuclear particles and membrane-bound messenger RNA.

Authors: L Cornudella; I Faiferman; A O Pogo
Journal: Biochim Biophys Acta Date: 1973-02-04

3. Some properties of RNA:protein complexes from the nucleus of eukaryotic cells.

Authors: T Martin; P Billings; A Levey; S Ozarslan; T Quinlan; H Swift; L Urbas
Journal: Cold Spring Harb Symp Quant Biol Date: 1974

4. In vitro synthesis of DNA complementary to rabbit reticulocyte 10S RNA.

Authors: I M Verma; G F Temple; H Fan; D Baltimore
Journal: Nat New Biol Date: 1972-02-09

5. Two classes of messenger RNA in cultured rat cells: repetitive sequence transcripts and unique sequence transcripts.

Authors: M S Campo; J O Bishop
Journal: J Mol Biol Date: 1974-12-25 Impact factor: 5.469

6. Proteins associated with heterogeneous nuclear RNA in eukaryotic cells.

Authors: T Pederson
Journal: J Mol Biol Date: 1974-02-25 Impact factor: 5.469

7. A simple general method to determine the proportion of active ribosomes in eukaryotic cells.

Authors: T E Martin
Journal: Exp Cell Res Date: 1973-08 Impact factor: 3.905

8. Kinetics of renaturation of DNA.

Authors: J G Wetmur; N Davidson
Journal: J Mol Biol Date: 1968-02-14 Impact factor: 5.469

9. The diversity of the messenger RNA population in growing Friend cells.

Authors: G D Birnie; E MacPhail; B D Young; M J Getz; J Paul
Journal: Cell Differ Date: 1974-11

10. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.

Authors: H Aviv; P Leder
Journal: Proc Natl Acad Sci U S A Date: 1972-06 Impact factor: 11.205

9 in total

1. A comparative study on the two classes of heterogeneous nuclear ribonucleoprotein particles separated in metrizamide density gradient, by electrophoresis of proteins and chase experiments. Evidence for two distinct subfractions of HnRNP in mammalian nuclei.

Authors: J F Houssais
Journal: Mol Biol Rep Date: 1977-03 Impact factor: 2.316

9. Structure of nuclear ribonucleoprotein: identification of proteins in contact with poly(A)+ heterogeneous nuclear RNA in living HeLa cells.

Authors: S Mayrand; B Setyono; J R Greenberg; T Pederson
Journal: J Cell Biol Date: 1981-08 Impact factor: 10.539

9 in total

Detection of mRNA sequences in nuclear 30S ribonucleoprotein subcomplexes.

1. Proteins associated with globin messenger RNA in avian erythroblasts: Isolation and comparison with the proteins bound to nuclear messenger-likie RNA.

2. Polyadenylic acid sequences in ascites cells nuclear particles and membrane-bound messenger RNA.

3. Some properties of RNA:protein complexes from the nucleus of eukaryotic cells.

4. In vitro synthesis of DNA complementary to rabbit reticulocyte 10S RNA.

5. Two classes of messenger RNA in cultured rat cells: repetitive sequence transcripts and unique sequence transcripts.

6. Proteins associated with heterogeneous nuclear RNA in eukaryotic cells.

7. A simple general method to determine the proportion of active ribosomes in eukaryotic cells.

8. Kinetics of renaturation of DNA.

9. The diversity of the messenger RNA population in growing Friend cells.

10. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.

1. A comparative study on the two classes of heterogeneous nuclear ribonucleoprotein particles separated in metrizamide density gradient, by electrophoresis of proteins and chase experiments. Evidence for two distinct subfractions of HnRNP in mammalian nuclei.

2. Poly(A+)mRNA sequences in free mRNP and polysomes of mouse ascites cells.

3. Structure of premRNP. Models and pitfalls.

4. Arrangement of 30S heterogeneous nuclear ribonucleoprotein on polyoma virus late nuclear transcripts.

5. HnRNP particles.

6. Relatively stable population of c-myc RNA that lacks long poly(A).

7. Preformed Messenger RNAs and Early Wheat Embryo Germination.

8. In vitro assembly of a pre-messenger ribonucleoprotein.

9. Structure of nuclear ribonucleoprotein: identification of proteins in contact with poly(A)+ heterogeneous nuclear RNA in living HeLa cells.