Literature DB >> 10666445

A simple in vitro Tn7-based transposition system with low target site selectivity for genome and gene analysis.

M C Biery1, F J Stewart, A E Stellwagen, E A Raleigh, N L Craig.   

Abstract

A robust Tn7-based in vitro transposition system is described that displays little target site selectivity, allowing the efficient recovery of many different transposon insertions in target DNAs ranging from small plasmids to cosmids to whole genomes. Two miniTn7 derivatives are described that are useful for the analysis of genes: one a derivative for making translational and transcriptional target gene fusions and the other a derivative that can generate 15 bp (5 amino acid) insertions in target DNAs (proteins).

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Year:  2000        PMID： 10666445      PMCID： PMC102592          DOI： 10.1093/nar/28.5.1067

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  29 in total

1.  In vitro transposition of Tn552: a tool for DNA sequencing and mutagenesis.

Authors:  T J Griffin; L Parsons; A E Leschziner; J DeVost; K M Derbyshire; N D Grindley
Journal:  Nucleic Acids Res       Date:  1999-10-01       Impact factor: 16.971

2.  An efficient and accurate integration of mini-Mu transposons in vitro: a general methodology for functional genetic analysis and molecular biology applications.

Authors:  S Haapa; S Taira; E Heikkinen; H Savilahti
Journal:  Nucleic Acids Res       Date:  1999-07-01       Impact factor: 16.971

3.  Host proteins can stimulate Tn7 transposition: a novel role for the ribosomal protein L29 and the acyl carrier protein.

Authors:  P L Sharpe; N L Craig
Journal:  EMBO J       Date:  1998-10-01       Impact factor: 11.598

4.  Systematic identification of essential genes by in vitro mariner mutagenesis.

Authors:  B J Akerley; E J Rubin; A Camilli; D J Lampe; H M Robertson; J J Mekalanos
Journal:  Proc Natl Acad Sci U S A       Date:  1998-07-21       Impact factor: 11.205

5.  Tn5/IS50 target recognition.

Authors:  I Y Goryshin; J A Miller; Y V Kil; V A Lanzov; W S Reznikoff
Journal:  Proc Natl Acad Sci U S A       Date:  1998-09-01       Impact factor: 11.205

Review 6.  Insertion sequences.

Authors:  J Mahillon; M Chandler
Journal:  Microbiol Mol Biol Rev       Date:  1998-09       Impact factor: 11.056

Review 7.  Mobile DNA elements: controlling transposition with ATP-dependent molecular switches.

Authors:  A E Stellwagen; N L Craig
Journal:  Trends Biochem Sci       Date:  1998-12       Impact factor: 13.807

Review 8.  Transposon Tn7.

Authors:  N L Craig
Journal:  Curr Top Microbiol Immunol       Date:  1996       Impact factor: 4.291

9.  Efficient integration of artificial transposons into plasmid targets in vitro: a useful tool for DNA mapping, sequencing and genetic analysis.

Authors:  S E Devine; J D Boeke
Journal:  Nucleic Acids Res       Date:  1994-09-11       Impact factor: 16.971

10.  Tn7 transposition: target DNA recognition is mediated by multiple Tn7-encoded proteins in a purified in vitro system.

Authors:  R J Bainton; K M Kubo; J N Feng; N L Craig
Journal:  Cell       Date:  1993-03-26       Impact factor: 41.582
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  54 in total

1.  Construction of gene-targeting vectors: a rapid Mu in vitro DNA transposition-based strategy generating null, potentially hypomorphic, and conditional alleles.

Authors:  H Vilen; S Eerikäinen; J Tornberg; M S Airaksinen; H Savilahti
Journal:  Transgenic Res       Date:  2001       Impact factor: 2.788

2.  Isolation and characterization of Tn7 transposase gain-of-function mutants: a model for transposase activation.

Authors:  F Lu; N L Craig
Journal:  EMBO J       Date:  2000-07-03       Impact factor: 11.598

3.  Formation of a nucleoprotein complex containing Tn7 and its target DNA regulates transposition initiation.

Authors:  Zachary Skelding; Robert Sarnovsky; Nancy L Craig
Journal:  EMBO J       Date:  2002-07-01       Impact factor: 11.598

4.  Comprehensive mutational analysis of a herpesvirus gene in the viral genome context reveals a region essential for virus replication.

Authors:  Anja Bubeck; Markus Wagner; Zsolt Ruzsics; Mark Lötzerich; Margot Iglesias; Ila R Singh; Ulrich H Koszinowski
Journal:  J Virol       Date:  2004-08       Impact factor: 5.103

5.  Global transposon mutagenesis and essential gene analysis of Helicobacter pylori.

Authors:  Nina R Salama; Benjamin Shepherd; Stanley Falkow
Journal:  J Bacteriol       Date:  2004-12       Impact factor: 3.490

6.  Revealing domain structure through linker-scanning analysis of the murine leukemia virus (MuLV) RNase H and MuLV and human immunodeficiency virus type 1 integrase proteins.

Authors:  Jennifer Puglia; Tan Wang; Christine Smith-Snyder; Marie Cote; Michael Scher; Joelle N Pelletier; Sinu John; Colleen B Jonsson; Monica J Roth
Journal:  J Virol       Date:  2006-10       Impact factor: 5.103

7.  Gene disruption in Cryptococcus neoformans and Cryptococcus gattii by in vitro transposition.

Authors:  Guanggan Hu; James W Kronstad
Journal:  Curr Genet       Date:  2006-01-06       Impact factor: 3.886

8.  Functional domains of murine cytomegalovirus nuclear egress protein M53/p38.

Authors:  Mark Lötzerich; Zsolt Ruzsics; Ulrich H Koszinowski
Journal:  J Virol       Date:  2006-01       Impact factor: 5.103

9.  Ku interacts with telomerase RNA to promote telomere addition at native and broken chromosome ends.

Authors:  Anne E Stellwagen; Zara W Haimberger; Joshua R Veatch; Daniel E Gottschling
Journal:  Genes Dev       Date:  2003-09-15       Impact factor: 11.361

10.  Domain structure of HrpE, the Hrp pilus subunit of Xanthomonas campestris pv. vesicatoria.

Authors:  Ernst Weber; Ralf Koebnik
Journal:  J Bacteriol       Date:  2005-09       Impact factor: 3.490
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