Literature DB >> 10664966

Production and characterization of monoclonal antibodies for aflatoxin B1.

K T Devi1, M A Mayo, K L Reddy, P Delfosse, G Reddy, S V Reddy, D V Reddy.   

Abstract

Hybridomas that secreted antibodies for aflatoxin B1 were selected using two immunization protocols referred to as A and B. Protocol A is a standard immunization method and resulted in the selection of only two clones that produced monoclonal antibodies against aflatoxin B1. In protocol B a unique immunization schedule which resulted in the generation of 10 hybridomas is described. Of the 10, one antibody was highly specific to B1, four antibodies reacted equally strongly with B1, G1 and weakly with B2. Another four reacted strongly with B1 and weakly with B2 and G1. One clone reacted equally strongly with B1, G1 and B2. Interestingly all the 10 antibodies showed little or no cross-reaction with G2.

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Year:  1999        PMID: 10664966     DOI: 10.1046/j.1472-765x.1999.00685.x

Source DB:  PubMed          Journal:  Lett Appl Microbiol        ISSN: 0266-8254            Impact factor:   2.858


  8 in total

1.  Highly Sensitive FRET-Based Fluorescence Immunoassay for Detecting of Aflatoxin B1 Using Magnetic/Silica Core-Shell as a Signal Intensifier.

Authors:  Alireza Kalarestaghi; Mansour Bayat; Seyed Jamal Hashemi; Vadood Razavilar
Journal:  Iran J Biotechnol       Date:  2015-09       Impact factor: 1.671

2.  A preliminary study on the occurrence of Aspergillus spp. and aflatoxin B1 in imported wheat and barley in Penang, Malaysia.

Authors:  K R N Reddy; Baharuddin Salleh
Journal:  Mycotoxin Res       Date:  2010-07-27       Impact factor: 3.833

3.  Novel aflatoxin derivatives and protein conjugates.

Authors:  Christian Cervino; Dietmar Knopp; Michael G Weller; Reinhard Niessner
Journal:  Molecules       Date:  2007-03-27       Impact factor: 4.411

Review 4.  Recent trends in rapid environmental monitoring of pathogens and toxicants: potential of nanoparticle-based biosensor and applications.

Authors:  Preeyaporn Koedrith; Thalisa Thasiphu; Jong-Il Weon; Rattana Boonprasert; Kooranee Tuitemwong; Pravate Tuitemwong
Journal:  ScientificWorldJournal       Date:  2015-03-25

Review 5.  Recent Progress in Rapid Determination of Mycotoxins Based on Emerging Biorecognition Molecules: A Review.

Authors:  Yanru Wang; Cui Zhang; Jianlong Wang; Dietmar Knopp
Journal:  Toxins (Basel)       Date:  2022-01-20       Impact factor: 4.546

6.  Monoclonal IgA Antibodies for Aflatoxin Immunoassays.

Authors:  Özlem Ertekin; Şerife Şeyda Pirinçci; Selma Öztürk
Journal:  Toxins (Basel)       Date:  2016-05-12       Impact factor: 4.546

7.  Label Free QCM Immunobiosensor for AFB1 Detection Using Monoclonal IgA Antibody as Recognition Element.

Authors:  Özlem Ertekin; Selma Öztürk; Zafer Ziya Öztürk
Journal:  Sensors (Basel)       Date:  2016-08-11       Impact factor: 3.576

Review 8.  Advances in Antibody Preparation Techniques for Immunoassays of Total Aflatoxin in Food.

Authors:  Yanan Wang; Jinqing Jiang; Hanna Fotina; Haitang Zhang; Junjie Chen
Journal:  Molecules       Date:  2020-09-09       Impact factor: 4.411

  8 in total

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