Literature DB >> 1065876

Temporal relationships of chromatin protein synthesis, DNA synthesis, and assembly of deoxyribonucleoprotein.

R L Seale.   

Abstract

Chromatin assembly has been investigated in terms of the sites on DNA where newly synthesized chromatin proteins associate. Chromatin from cells labeled with [14C]-BrdUrd and [3H]lysine was fixed with formaldehyde and resolved in CsCl gradients. By varying the spacing of the labeling intervals of the two isotopes so as to encompass all possible periods in S-phase, the association of labeled, newly synthesized proteins on newly synthesized (BrdUrd-substituted) or preexisting chromatin DNA was determined. In all experiments it was found that newly synthesized chromatin proteins predominantly associated with nonreplicating DNA. Possible mechanisms by which cells recycle preexisting chromatin proteins to restore the protein content of newly synthesized DNA are discussed.

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Year:  1976        PMID： 1065876      PMCID： PMC430526          DOI： 10.1073/pnas.73.7.2270

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  36 in total

1.  DNA synthesis in human lymphocyts: intermediates in DNA synthesis, in vitro and in vivo.

Authors:  B Y Tseng; M Goulian
Journal:  J Mol Biol       Date:  1975-12-05       Impact factor: 5.469

2.  Effects of cycloheximide on chromatin biosynthesis.

Authors:  R L Seale; R T Simpson
Journal:  J Mol Biol       Date:  1975-05-25       Impact factor: 5.469

3.  Assembly of DNA and protein during replication in HeLa cells.

Authors:  R L Seale
Journal:  Nature       Date:  1975-05-15       Impact factor: 49.962

4.  Kinetics of inactivation of histone mRNA in the cytoplasm after inhibition of DNA replication in synchronised HeLa cells.

Authors:  D Gallwitz
Journal:  Nature       Date:  1975-09-18       Impact factor: 49.962

5.  The organization of proteins in polyoma and cellular chromatin.

Authors:  A J Louie
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1975

6.  Deposition of histones onto replicating chromosomes.

Authors:  V Jackson; D K Granner; R Chalkley
Journal:  Proc Natl Acad Sci U S A       Date:  1975-11       Impact factor: 11.205

7.  Polyoma virus basic proteins.

Authors:  P M Frearson; L V Crawford
Journal:  J Gen Virol       Date:  1972-02       Impact factor: 3.891

8.  Characterization of chromatin extensively substituted with 5-bromodeoxyuridine.

Authors:  R T Simpson; R L Seale
Journal:  Biochemistry       Date:  1974-10-22       Impact factor: 3.162

9.  Dna synthesis in isolated HeLa nuclei. A system for continuation of replication in vivo.

Authors:  H V Hershey; J F Stieber; G C Mueller
Journal:  Eur J Biochem       Date:  1973-04

10.  The units of DNA replication in Drosophila melanogaster chromosomes.

Authors:  A B Blumenthal; H J Kriegstein; D S Hogness
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1974
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  12 in total

1.  Deposition of histone onto the replicating chromosome: newly synthesized histone is not found near the replication fork.

Authors:  V Jackson; D Granner; R Chalkley
Journal:  Proc Natl Acad Sci U S A       Date:  1976-07       Impact factor: 11.205

Review 2.  Chromatin replication revealed by studies of animal cells and papovaviruses (simian virus 40 and polyoma virus).

Authors:  C Crémisi
Journal:  Microbiol Rev       Date:  1979-09

3.  Nucleosomes associated with newly replicated DNA have an altered conformation.

Authors:  R L Seale
Journal:  Proc Natl Acad Sci U S A       Date:  1978-06       Impact factor: 11.205

4.  The use of ultraviolet light in the fractionation of chromatin containing unsubstituted and bromodeoxyuridine-substituted DNA.

Authors:  L B Taichman
Journal:  Nucleic Acids Res       Date:  1979       Impact factor: 16.971

5.  Isolation of newly replicated chromatin by using shallow metrizamide gradients.

Authors:  R F Murphy; R B Wallace; J Bonner
Journal:  Proc Natl Acad Sci U S A       Date:  1980-06       Impact factor: 11.205

6.  The sites of deposition of newly synthesized histone.

Authors:  V Jackson; S Marshall; R Chalkley
Journal:  Nucleic Acids Res       Date:  1981-09-25       Impact factor: 16.971

7.  Histones and DNA increase synchronously in neurons during early postnatal development of the rat forebrain cortex.

Authors:  A Bregnard; F Ruch; H Lutz; C C Kuenzle
Journal:  Histochemistry       Date:  1979-07-11

8.  Assembly of new histones into nucleosomes and their distribution in replicating chromatin.

Authors:  G Russev; R Hancock
Journal:  Proc Natl Acad Sci U S A       Date:  1982-05       Impact factor: 11.205

9.  Distribution of the core histones H2A.H2B.H3 and H4 during cell replication.

Authors:  E Fowler; R Farb; S El-Saidy
Journal:  Nucleic Acids Res       Date:  1982-01-22       Impact factor: 16.971

10.  Preferential binding of benzo[a]pyrene diol epoxide to the linker DNA of human foreskin fibroblasts in S phase in the presence of benzamide.

Authors:  P Kurian; A M Jeffrey; G E Milo
Journal:  Proc Natl Acad Sci U S A       Date:  1985-05       Impact factor: 11.205
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