Y G Yang1, A M Chen, J J Sergio, Y Zhou, M Sykes. 1. Bone Marrow Transplantation Section, Transplantation Biology Research Center, Massachusetts General Hospital/Harvard Medical School, Boston 02129, USA.
Abstract
BACKGROUND: Although complement activation has been shown to be important in the rejection of solid organs in some xenogeneic species combinations, its role in the rejection of xenogeneic marrow engraftment is unknown. METHODS: The effect of complement depletion with cobra venom factor on porcine bone marrow cell (BMC) engraftment was examined in 3 Gy-irradiated C.B-17 severe combined immunodeficiency mice receiving 10(8) pig BMC. RESULTS: At 26 days after transplantation, the percentages of swine class I+, myeloid, and CD2+ cells in marrow, spleen, and peripheral blood, and the numbers of porcine myeloid progenitor cells in marrow, were increased in cobra venom factor-treated recipients compared with simultaneous control recipients. Consistent with the in vivo results, preheating serum (56 degrees C for 30 min) reduced the inhibitory effect of severe combined immunodeficiency mouse serum on the proliferation of pig BMC in vitro. CONCLUSION: Murine complement is capable of resisting xenogeneic hematopoietic engraftment through an antibody-independent mechanism.
BACKGROUND: Although complement activation has been shown to be important in the rejection of solid organs in some xenogeneic species combinations, its role in the rejection of xenogeneic marrow engraftment is unknown. METHODS: The effect of complement depletion with cobra venom factor on porcine bone marrow cell (BMC) engraftment was examined in 3 Gy-irradiated C.B-17 severe combined immunodeficiencymice receiving 10(8) pig BMC. RESULTS: At 26 days after transplantation, the percentages of swine class I+, myeloid, and CD2+ cells in marrow, spleen, and peripheral blood, and the numbers of porcine myeloid progenitor cells in marrow, were increased in cobra venom factor-treated recipients compared with simultaneous control recipients. Consistent with the in vivo results, preheating serum (56 degrees C for 30 min) reduced the inhibitory effect of severe combined immunodeficiencymouse serum on the proliferation of pig BMC in vitro. CONCLUSION:Murine complement is capable of resisting xenogeneic hematopoietic engraftment through an antibody-independent mechanism.