Literature DB >> 10651287

An indexed genomic library for Paramecium complementation cloning.

A M Keller1, J Cohen.   

Abstract

Recent pioneering work opened the way for cloning genes in Paramecium by functional complementation of mutants. We present here the construction and pilot utilization of a new indexed library of Paramecium macronuclear DNA. The library is made of 61,440 clones containing inserts mostly between 6 and 12 kilobases. It has already allowed the complementation cloning of four new genes, and this library has proven to be very useful for rapid hybridization cloning.

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Year:  2000        PMID: 10651287     DOI: 10.1111/j.1550-7408.2000.tb00001.x

Source DB:  PubMed          Journal:  J Eukaryot Microbiol        ISSN: 1066-5234            Impact factor:   3.346


  14 in total

1.  PAK paradox: Paramecium appears to have more K(+)-channel genes than humans.

Authors:  W John Haynes; Kit-Yin Ling; Yoshiro Saimi; Ching Kung
Journal:  Eukaryot Cell       Date:  2003-08

2.  Nd6p, a novel protein with RCC1-like domains involved in exocytosis in Paramecium tetraurelia.

Authors:  Delphine Gogendeau; Anne-Marie Keller; Akira Yanagi; Jean Cohen; France Koll
Journal:  Eukaryot Cell       Date:  2005-12

3.  Analysis of sequence variability in the macronuclear DNA of Paramecium tetraurelia: a somatic view of the germline.

Authors:  Laurent Duret; Jean Cohen; Claire Jubin; Philippe Dessen; Jean-François Goût; Sylvain Mousset; Jean-Marc Aury; Olivier Jaillon; Benjamin Noël; Olivier Arnaiz; Mireille Bétermier; Patrick Wincker; Eric Meyer; Linda Sperling
Journal:  Genome Res       Date:  2008-02-06       Impact factor: 9.043

Review 4.  What do genic mutations tell us about the structural patterning of a complex single-celled organism?

Authors:  Joseph Frankel
Journal:  Eukaryot Cell       Date:  2008-07-25

5.  An exchanger-like protein underlies the large Mg2+ current in Paramecium.

Authors:  W John Haynes; Ching Kung; Yoshiro Saimi; Robin R Preston
Journal:  Proc Natl Acad Sci U S A       Date:  2002-11-06       Impact factor: 11.205

6.  Random sequencing of Paramecium somatic DNA.

Authors:  Linda Sperling; Philippe Dessen; Marek Zagulski; Ron E Pearlman; Andrzey Migdalski; Robert Gromadka; Marine Froissard; Anne-Marie Keller; Jean Cohen
Journal:  Eukaryot Cell       Date:  2002-06

7.  Multigene family encoding 3',5'-cyclic-GMP-dependent protein kinases in Paramecium tetraurelia cells.

Authors:  Roland Kissmehl; Tim P Krüger; Tilman Treptau; Marine Froissard; Helmut Plattner
Journal:  Eukaryot Cell       Date:  2006-01

8.  Core formation and the acquisition of fusion competence are linked during secretory granule maturation in Tetrahymena.

Authors:  Grant R Bowman; Nels C Elde; Garry Morgan; Mark Winey; Aaron P Turkewitz
Journal:  Traffic       Date:  2005-04       Impact factor: 6.215

9.  Novel types of Ca2+ release channels participate in the secretory cycle of Paramecium cells.

Authors:  Eva-Maria Ladenburger; Ivonne M Sehring; Iris Korn; Helmut Plattner
Journal:  Mol Cell Biol       Date:  2009-04-20       Impact factor: 4.272

10.  ParameciumDB: a community resource that integrates the Paramecium tetraurelia genome sequence with genetic data.

Authors:  Olivier Arnaiz; Scott Cain; Jean Cohen; Linda Sperling
Journal:  Nucleic Acids Res       Date:  2006-11-16       Impact factor: 16.971

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