| Literature DB >> 10650913 |
T Brüning1, J Abel, B Koch, K Lorenzen, V Harth, S Donat, A Sachinidis, H Vetter, H M Bolt, Y Ko.
Abstract
The aim of the present study was to develop and validate a rapid assay for genotyping of CYP1B1 codon 432-polymorphism. The described method is a single tube assay and combines both rapid-cycle polymerase chain reaction (PCR) with real-time monitoring by amplification and generation of the melting profiles of an allele-specific fluorescent probe. With this method 300 samples were analysed from healthy, unrelated Germans. Genotype frequency determined for the mutated allele CYP1B1*2 was 0.40. The results show that genotyping of CYP1B1 codon 432-polymorphism with a real-time fluorescence PCR method is a rapid and reliable assay for the analysis of large numbers of samples.Entities:
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Year: 1999 PMID： 10650913 DOI： 10.1007/s002040050631
Source DB: PubMed Journal: Arch Toxicol ISSN： 0340-5761 Impact factor: 5.153